ENAGenomicsUniversity of Wisconsin - Madisonhttps://www.ebi.ac.uk/ena/browser/view/PRJNA176148xref:PubMed:23818864Mapping the occupancy of FNR, HNS, and IHF throughout the genome of Escherchia coli MG1655 K-12 using an affinity purified antibody under anerobic growth conditions. We also mapped the binding of the ß subunit of RNA Polymerase under both aerobic and anaerobic growth conditions. As a control, we also performed ChIP-chip on FNR in a ∆fnr mutant strain of Escherchia coli MG1655 K-12. We also examined FNR immunoprecipitation at various FNR concentrations using IPTG and Ptac::fnr (PK8263). The ∆hns/∆stpA strains were also used. Descirbed in the manuscript Genome-scale Analysis of E. coli FNR Reveals the Complexity of Bacterial Regulon Structure Overall design: Mapping of occupancy of FNR, NNS, IHF and ß of RNAP in the genome of Escherchia coli MG1655 K-12 under aerobic or anaerobic growth conditions. Immunoprecipitated DNA compared to INPUT for each sample.ENAHITS-CLIP, High Throughput Sequencing of RNA Isolated by Crosslinking Immunoprecipitation, Chromatin Immunoprecipitation Sequencing Chip, ChIP-Chip, Chromatin Immuno precipitation Sequencing, Escherichia coli strain MG1655, Chromatin Immuno-precipitation, ChIP, Chromatin Immunoprecipitation Paired End Tag, Cross Linking and Immunoprecipitation Followed by Deep Sequencing, Chromatin Immuno Precipitation Paired End Tag, Sprain, Cross-Linking and Immunoprecipitation Followed by Deep Sequencing, ChIP Sequencing, Chromatin Immunoprecipitation, CLIP-Seq, Chromatin Immuno-precipitation Sequencing, Escherichia coli str. MG1655, Assay for Transposase-Accessible Chromatin Using Sequencing, ChIP Exonuclease, Escherichia coli str. K12 substr. MG1655, ChIP-PET, ChIP-Seq, Sequencing, ChIP-Exo, Assay for Transposase Accessible Chromatin Using Sequencing, Chromatin Immunoprecipitation Paired-End Tag, Escherichia coli MG1655, Chromatin Immuno-Precipitation Paired-End Tag, ATAC-Seq, Chromatin Immunoprecipitation Sequencing-Chips, Chromatin Immunoprecipitation Sequencing-Chip, Strain., High-Throughput Sequencing of RNA Isolated by Crosslinking Immunoprecipitation, Strains, ChIP-Exonuclease, Sprains, Strains and Sprains, ChIA-PETHITS-CLIP, High Throughput Sequencing of RNA Isolated by Crosslinking Immunoprecipitation, Chromatin Immunoprecipitation Sequencing Chip, ChIP-Chip, Chromatin Immuno precipitation Sequencing, Escherichia coli strain MG1655, Chromatin Immuno-precipitation, ChIP, Chromatin Immunoprecipitation Paired End Tag, Cross Linking and Immunoprecipitation Followed by Deep Sequencing, Chromatin Immuno Precipitation Paired End Tag, Sprain, Cross-Linking and Immunoprecipitation Followed by Deep Sequencing, ChIP Sequencing, Chromatin Immunoprecipitation, CLIP-Seq, Chromatin Immuno-precipitation Sequencing, Escherichia coli str. MG1655, Assay for Transposase-Accessible Chromatin Using Sequencing, ChIP Exonuclease, Escherichia coli str. K12 substr. MG1655, ChIP-PET, ChIP-Seq, Sequencing, ChIP-Exo, Assay for Transposase Accessible Chromatin Using Sequencing, Chromatin Immunoprecipitation Paired-End Tag, Escherichia coli MG1655, Chromatin Immuno-Precipitation Paired-End Tag, ATAC-Seq, Chromatin Immunoprecipitation Sequencing-Chips, Chromatin Immunoprecipitation Sequencing-Chip, Strain., High-Throughput Sequencing of RNA Isolated by Crosslinking Immunoprecipitation, Strains, ChIP-Exonuclease, Sprains, Strains and Sprains, ChIA-PETfalseChip-chip from Escherichia coli MG1655 K-12, WT and ∆fnr strainsChip-chip from Escherichia coli MG1655 K-12, WT and ∆fnr strains2025-09-242014-02-11PRJNA176148GSE4118623818864