{"database":"ENA","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Fastqsanger.gz":["ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR621/SRR621139/SRR621139.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR621/SRR621138/SRR621138.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR621/SRR621140/SRR621140.fastq.gz"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Genomics"],"center_name":["Krogan, Cellular & Molecular Pharmacology, University of California, San Francisco"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJNA182318"],"scientific_name":["Homo sapiens"],"tag":["xref:PubMed:23333304"],"long_description":["The HIV-1 accessory protein Vif hijacks a cellular Cullin-RING ubiquitin ligase, CRL5, to promote degradation of the APOBEC3 (A3) family of restriction factors. Recently, the cellular transcription cofactor CBFb was shown to form a complex with CRL5-Vif and to be essential for A3 degradation and viral infectivity. We now demonstrate that CBFb is required for assembling a well-ordered CRL5-Vif complex by inhibiting Vif oligomerization and by activating CRL5-Vif via direct interaction. The CRL5-Vif-CBFb holoenzyme forms a welldefined heterohexamer, indicating that Vif simultaneously hijacks CRL5 and CBFb. Heterodimers of CBFb and RUNX transcription factors contribute toward the regulation of genes, including those with immune system functions. We show that binding of Vif to CBFb is mutually exclusive with RUNX heterodimerization and impacts the expression of genes whose regulatory domains are associated with RUNX1. Our results provide a mechanism by which a pathogen with limited coding capacity uses one factor to hijack multiple host pathways. Overall design: Identification of RUNX1 binding sites in the Jurkat cell line"],"repository":["ENA"],"description_synonyms":["Human T-Cell Lymphotropic Virus Type III, ChIP-Chip, Chromatin Immuno-precipitation, AIDS Virus, HTLV-III, Gene, CLIP-Seq, Assay for Transposase-Accessible Chromatin Using Sequencing, Human Immunodeficiency, Human, Lymphadenopathy Associated Virus, Cbfa2, Chromatin Immunoprecipitation Sequencing-Chip, Virus, Pebpb2, Human T-Cell Leukemia Virus Type III, Human immunodeficiency virus, Runx-1, Chromatin Immunoprecipitation Sequencing Chip, CBFB, Human T-Lymphotropic Virus Type III, Pebp2a2, Chromatin Immuno precipitation Sequencing, Gene Expressions, pea2-beta, Viruses, AMLCR1, ChIP, Chromatin Immunoprecipitation Paired End Tag, Chromatin Immuno Precipitation Paired End Tag, Cross-Linking and Immunoprecipitation Followed by Deep Sequencing, Immunodeficiency Viruses, cbfbeta, amlcr1, Human T Cell Leukemia Virus Type III, Expressions, pebp2ab, aml1, Sequencing, XAML, Chromatin Immunoprecipitation Paired-End Tag, pebp2-beta, Pebpa2b, pebp2b, ATAC-Seq, Chromatin Immunoprecipitation Sequencing-Chips, Lymphadenopathy-Associated Viruses, CBF-B, HIV, Expression, Human Immunodeficiency Viruses, Lymphadenopathy-Associated Virus, HITS-CLIP, High Throughput Sequencing of RNA Isolated by Crosslinking Immunoprecipitation, Human Immunodeficiency Virus, CBF-alpha-2, Human T Lymphotropic Virus Type III, Aml1, AML1, Cross Linking and Immunoprecipitation Followed by Deep Sequencing, ChIP Sequencing, ChIP-PET, ChIP-Exo, EVI-1, PEBP2aB, AIDS, Immunodeficiency Virus, AI462102, High-Throughput Sequencing of RNA Isolated by Crosslinking Immunoprecipitation, aml1-evi-1, cbfa2, evi-1, AIDS virus, Acquired Immune Deficiency Syndrome Virus, aml, AML1-EVI-1, Nfya, Lymphadenopathy-Associated, AIDS Viruses, Xaml1, Chromatin Immunoprecipitation, Chromatin Immuno-precipitation Sequencing, CBFA2, ChIP Exonuclease, ChIP-Seq, PEBP2b, LAV-HTLV-III, Assay for Transposase Accessible Chromatin Using Sequencing, PEA2, ChIA-PET., Chromatin Immuno-Precipitation Paired-End Tag, Acquired Immunodeficiency Syndrome Virus, aml-1, AI893578, Pebp2, ChIP-Exonuclease, cbf-beta, PEBP2B, Human T Cell Lymphotropic Virus Type III, human immunodeficiency virus infectious disease"],"name_synonyms":["Human, Modern., human being, Man (Taxonomy), Homo sapiens, man, Man, human, Modern Man"],"additional_accession":[]},"is_claimable":false,"name":"Homo sapiens","description":"CBFb Stabilizes HIV Vif to Counteract APOBEC3 at the Expense of RUNX1 Target Gene Expression [ChIP-seq]","dates":{"last_updated":"2025-09-24","first_public":"2013-05-31"},"accession":"PRJNA182318","cross_references":{"GEO":["GSE42575"],"taxon":["9606"],"PubMed":["23333304"]}}