ENAGenomicsBioinformatics, Genentech, Inc.https://www.ebi.ac.uk/ena/browser/view/PRJNA195417Mus musculusxref:PubMed:24141388Innate type-2 lymphocytes (ILC2s) are a newly described cell type whose biology and contribution to disease are poorly understood. We are interested in investigating the role of the transcription factor Gfi1 in ILC2s, which appear to be a prominent source of IL-5 and IL-13 during type-2 immune responses. We have compelling evidence demonstrating a critical role for Gfi1 in the development and effector state of ILC2s. We would like to elucidate the molecular role of Gfi1 in ILC2s by identifying Gfi1-regulated genes by microarray analysis. Overall design: Gfi1+/+ or Gfi1GFP/GFP (a GFP cassette has been 'knocked-in' to the Gfi1 locus and thus functions both as a surrogate for Gfi1 promoter activity and as a loss-of-function allele) mice were injected with IL-25 once daily for 4 days. On day 5, ILC2s (Lin-/Sca-1+/ICOS+/CD127+/c-Kit+) were sorted from the mesenteric lymph nodes of Gfi1+/+ (3 mice/sample were pooled together) or Gfi1-/- mice (6 mice/sample were pooled together) and cultured for 6 days in RPMI 1640, 10% FBS, Gln, P/S, 2ME, IL-2 (50ng/ml), IL-7 (10ng/ml), and IL-25 (50ng/ml). Whole RNA was harvested on day 6 via the RNeasy kit (Qiagen).ENAMGC130048, AW495828, SGCG_HUMAN, 35 kDa dystrophin-associated glycoprotein, Laboratory, Mus domesticus, ILC2, mouse, SG-gamma, metazoans, A4, Metazoa., animals, House Mouse, Cell, TYPE, SGCG, LGMD2C, DAGA4, ZNF163, House, Mus, 35DAG, nuocyte, Mus musculus domesticus, sarcoglycan, Pal1, Animal, MAM, gamma-SG, Mice, SCG3, Animalia, Mus musculus, gamma sarcoglycan, DMDA1, Pal-1, Swiss, mice, Swiss Mouse, SCN2, House Mice, Swiss Mice, gamma (35kDa dystrophin-associated glycoprotein), domesticus, Laboratory Mice, multicellular animals, DMDA, GFI-1, 35kD dystrophin-associated glycoprotein, SCARMD2, natural helper cell, gamma-sarcoglycan, GFI1A, Mouse, house mouse, Gfi-1, Laboratory MouseMus musculus, Laboratory Mice., House, Mus, Laboratory, Swiss, Mus domesticus, mouse, Mus musculus domesticus, Swiss Mouse, mouse <Mus musculus>, Mouse, House Mice, Swiss Mice, house mouse, Mice, Laboratory Mouse, House Mouse, mice C57BL/6xCBA/CaJ hybrid, domesticus, Mus muscarisfalseMus musculusMouse ILC2 cells from wild-type or Gfi1-KO animals2025-09-222014-02-11PRJNA195417GSE456211009024141388