ENAGenomicsNew Projects Development Division, Toray Industries,Inc.https://www.ebi.ac.uk/ena/browser/view/PRJNA197388Mus musculusSelf-renewal potential and multipotency are hallmarks of the stem cell1-3. It is generally conceived that acquisition of such a stemness requires rejuvenation of somatic cells through reprogramming of genetic and epigenetic status. We show here that a simple blockage of cell differentiation is sufficient to make stem cells. By over-expression of transcriptional repressor Id3 in murine hematopoitic progenitor cells and cultivation of these cells in B cell induction conditions, cells exhibit developmental arrest and enter a self-renewal cycle. These cells can be maintained in vitro almost limitlessly, and the long-term cultured cells exhibited robust multi-lineage reconstitution when transferred into irradiated mice. These cells can be cloned and re-expanded with 50% of plating efficiency, indicating that virtually all cells are self-renewing. Equivalent progenitors were produced from progenitors in human cord blood. Thus produced stem cells will be useful as the source of cells for immune cell therapy. Overall design: Four samplesENAepilepsy, ESES with language regression, Controlling, AA408400, acquired epileptiform aphasia, preventive therapy, Microarray Analytical, EESWAS, Tcfe2a, ALF2, E47, Nanoarray, Microarray, Devices, Nanoarray Analytical Device, continuous spike-wave in sleep, and speech dyspraxia, A1, mental retardation, continuous Spike and waves during slow-Wave sleep syndrome, xtcf-3, e2a, epileptic aphasia, E12|E47, ME2, autosomal dominant, Cell, E12/E47, acquired, prevention, eses index, aphasia, TCF-3, bhlhb21, continuous spikes and waves during slow-wave sleep, Device, Nanoarray Analytical, E12, Nanoarray Analytical Devices, electrographic status epilepticus in sleep, focal, ITF1, LKS, Analysis, Microarray Analytical Devices, epileptic encephalopathy with continuous spike-and-wave during slow sleep, prevention and control, Microchip, intellectual disability, AW209082, continuous spike-wave during slow sleep syndrome, with speech disorder and with or without intellectual disability, Microarray Microchip, itf1, tcfe2a, Rolandic epilepsy, reference sample, prophylaxis, epileptic encephalopathy with spike-and-wave activation in sleep, EE-SWAS, with epilepsy, Microchips, CSWS, E2A, Analytical Device, with speech disorder and with or without mental retardation, Analytical Devices, Microarray Analytical Device, benign epilepsy of childhood with centrotemporal spikes, vdir, acquired aphasia with convulsive disorder, preventive measures, CSWSS syndrome, xtcf3, Microarray Microchips, control, VDIR., epileptic encephalopathy with spike and wave activation in sleep, LK syndrome, bHLHb21, FESD, Pan2, Pan1, electrographic status epilepticus of sleep, electrical status epilepticus of sleep, continuous slow spike and wave of sleep, ControlledMus musculus, Laboratory Mice., House, Mus, Laboratory, Swiss, Mus domesticus, mouse, Mus musculus domesticus, Swiss Mouse, mouse <Mus musculus>, Mouse, House Mice, Swiss Mice, house mouse, Mice, Laboratory Mouse, House Mouse, mice C57BL/6xCBA/CaJ hybrid, domesticus, Mus muscarisfalseMus musculusMicroarray analysis of IdHP cells compared to LKS, Control ProB and E2A-/- progenitors2025-09-242014-06-01PRJNA197388GSE4615810090