ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR866/SRR866247/SRR866247_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR866/SRR866248/SRR866248_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR866/SRR866246/SRR866246_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR866/SRR866249/SRR866249_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR866/SRR866246/SRR866246_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR866/SRR866249/SRR866249_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR866/SRR866247/SRR866247_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR866/SRR866248/SRR866248_1.fastq.gzprimaryOK200GenomicsDeepak Srivastava, Gladstone Institute of Cardiovascular Disease, J. David Gladstone Instituteshttps://www.ebi.ac.uk/ena/browser/view/PRJNA205009Homo sapiensWe are interested in the role of NOTCH1 and Shear Stress in Aortic Valve Endothelium. Overall design: Primary human aortic valve endothelium was subjected to 4 conditions in vitro. 1) Control siRNA, No shear stress. 2) NOTCH1 siRNA, No shear stress. 3) Control siRNA, 15 dynes/cm2 shear stress. 4) NOTCH1 siRNA, 15 dynes/cm2 shear stress. Triplicates of each condition were pooled for library perp and sequencingENAfalseHomo sapiensShear Stress and NOTCH1 Regulate Calcification Related Genes in Human Aortic Valve Endothelium (RNA-Seq)2025-09-242015-05-20PRJNA205009GSE471579606