ENAGenomicsMOLECULAR DIAGNOSTIC LABORATORY, THOMAS JEFFERSON UNIVERSITY HOSPITALhttps://www.ebi.ac.uk/ena/browser/view/PRJNA212633Homo sapiensBackground and Aim: Liver cirrhosis is associated with decreased hepatic cytochrome P4503A (CYP3A) activity but the pathogenesis of this phenomenon is not well elucidated. In this study, we examined if certain microRNAs (miRNA) are associated with decreased hepatic CYP3A activity in cirrhosis. Hepatic CYP3A activity and miRNA microarray expression profiles were measured in cirrhotic (n=22) and normal (n=12) liver tissue. Hepatic CYP3A activity was measured via midazolam hydroxylation in human liver microsomes. Additionally, hepatic CYP3A4 protein concentration and the expression of CYP3A4 mRNA were measured. Analyses were conducted to identify miRNAs which were differentially expressed between two groups but also were significantly associated with lower hepatic CYP3A activity. Overall design: Liver tissue was collected from individuals with established cirrhosis who were awaiting liver transplantation (n=22) at the time of their liver transplantation procedure in the operating room. Normal liver tissue (n=12) was obtained from patients undergoing liver resection for metastatic/benign liver lesions with no underlying chronic liver diseasexref:PubMed:24058572ENAHuman, Modern., human being, Man (Taxonomy), Homo sapiens, man, Man, human, Modern ManA_IG005I10.21, F14M4.21, DL3735W, F6G17_50, F14M4.22, MJB24.3, POLYPEPTIDE 7, FAMILY 77, MJB24.7, A_IG005I10_21, T20B5_9, Small, MFB13_19, SUR2, Cytochrome P 450 Monooxygenase, F4P12.1, Cytochrome P 450 Oxygenase, F6G17_60, F14P1_4, T11J7.16, T11J7.14, F3L17.70, CIRRH, CYP77A7, MOJ9.6, F6G17.50, T11J7_16, Liver cirrhosis, T11J7_14, K24M7_14, MJJ3.9, ABERRANT INDUCTION OF TYPE THREE 1, CYP90A, F2P9_2, MFB13.19, MDN11.4, CYTOCHROME P450 79F2, DL3710C, K24M7.14, miRNA, P450 protein, F6G17.60, F3L17_70, T1E3_20, F6A4.110, decreased, Cytochrome P450, F22H5.19, T1E3.20, F6G17_80, CYTOCHROME P450, F13O11.20, F6A4.120, F13O11.25, F13O11.23, F13O11.24, T29H11.210, THALIAN-DIOL DESATURASE, CYP450 Family, T1E3.4, Cytochrome P-450 Superfamily, cytochrome P450 activity, F6G17.80, DWF3, DL3720W, Cirrhosis, T22C12_3, DL3600C, pre-miRNA, pri miRNA, CYP703, CYP89, miRNAs, F11C18.7, F11C18.9, F5G3_8, Cytochrome P 450 Enzymes, CBB3, T26C19.1, MFD22.6, F5M6.19, LUTEIN DEFICIENT 5, CYP90, T5J17.120, F13O11_20, T29H11.200, MicroRNA, F5M6_19, Cytochrome P-450 Enzyme, F13O11_24, F13O11_25, F13O11_23, K9E15_12, F6A4.160, T4C9.140, DWARF 3, T12H17.80, stRNA, K9E15.12, F1C12_160, F6A4.170, Cytochrome P-450 Families, K16E1.5, K16E1.6, T12H17_80, YUP8H12R.1, T12H17_100, RED ELONGATED 1, F12B17.50, F5A8_3, P-450 Enzymes, T4C9.160, liver cirrhosis., T29A15_200, T4C9.150, F21J6.102, Enzyme, SUBFAMILY A, T8K22_12, F6F3.8, F21J6.4, F21J6.2, F13D4_120, pre miRNA, K15C23.6, ALTERED TRYPTOPHAN REGULATION 4, RNA, F17K2.9, F17K2.8, polypeptide 25, LUT5, polypeptide 26, polypeptide 23, polypeptide 24, ATR4, polypeptide 21, polypeptide 22, polypeptide 20, K8K14_3, polypeptide 18, polypeptide 19, polypeptide 16, Primary, polypeptide 17, CYTOCHROME P450 MONOOXYGENASE 71B2, T8K22.12, family 735, subfamily K, subfamily H, F5I10.21, subfamily F, subfamily G, RUNT 1, T4C9.170, subfamily D, subfamily B, polypeptide 14, F3M18.13, subfamily C, polypeptide 15, polypeptide 12, subfamily A, P450 Enzymes, polypeptide 13, polypeptide 10, polypeptide 11, CYTOCHROME P450 MONOOXYGENASE, THAD, polypeptide 30 pseudogene, family 71 subfamily B, F3F19_13, KAO1, THAD1, P-450 Enzyme, T3A4.4, F3F19.10, F5I10_21, F3F19.11, F3F19.12, F3F19.13, RNT1, F1M20_23, RED1, Temporal RNA, ATT1, polypeptide 38, Superfamily, T20P8.6, F18A5.160, T20P8.5, polypeptide 36, polypeptide 37, polypeptide 34, cytochrome P450, polypeptide 35, polypeptide 32, polypeptide 33, T18B16_200, polypeptide 30, polypeptide 31, F1M20.23, F1M20.22, polypeptide 29, polypeptide 27, polypeptide 28, CYTOCHROME P450 MONOOXYGENASE 83B1, General activity, F1M20_22, F18A5_160, T12C24_27, F7D19.15, Activity, REDUCED EPIDERMAL FLUORESCENCE 2, Cytochrome P 450, MXI10.18, polypeptide 2, polypeptide 1, ENT-KAURENOIC ACID OXYDASE 1, T9E8.50, MOJ9_6, polypeptide 8, F7D19_15, polypeptide 7, F3O9_20, polypeptide 9, polypeptide 4, polypeptide 3, Monooxygenase, polypeptide 6, polypeptide 5, MXI10_18, T9E8_50, CYTOCHROME P450 MONOOXYGENASE 91A2, REF2, present in fewer numbers in organism, F3O9.20, family 703, F13P17.35, F2P9.2, family 707, family 706, family 705, F14P1.4, Small Temporal, UNFERTILIZED EMBRYO SAC 9, family 709, MDN11_4, SUPERROOT 2, T9I4.17, YUP8H12.23, F3F19_10, F25C20_25, F25C20_24, F3F19_12, F3F19_11, F3M18_13, Primary miRNA, family 710, MJJ3_9, INSDC_feature:ncRNA, pri-miRNA, CYP81F1, family 715, family 714, F13P17.22, F25C20.25, F25C20.24, family 712, family 718, T24P15_16, FCAALL.274, FCAALL.270, T20B5.9, T12C24.27, T24P15.16, family 722, YUP8H12_23, T9I4_17, family 721, MJB24_3, AT5G57250, MJB24_7, Cytochrome P 450 Enzyme System, F6A4_110, Cytochrome P450 Superfamily, Small Temporal RNA, P 450 Enzyme, CYP, F6A4_120, T22C12.3, Cytochrome P-450 Enzymes, P-450, F21F23_15, T5J17_120, T20D16.18, P450 Enzyme, T20D16.15, F5G3.8, T20D16_15, CYTOCHROME P450 90A1, FCAALL.208, F21F23.15, T20D16_18, Micro, F12B17_50, F12B17.40, K16E1_6, K16E1_5, Cytochrome p450 Families, T9E8.30, F12B17_40, T9E8_30, CYTOCHROME P450 MONOOXYGENASE 81F1, Cytochrome, Cytochrome P-450 Oxygenase, F17K2.10, F17K2.11, CABBAGE 3, KLU, MYH9.18, reduced, Cytochrome P 450 Dependent Monooxygenase, subnumerary, T18B16.200, T4C9_140, F13D4.120, Fibroses, F5A8.3, MYH9_18, F6A4_160, Cytochrome P-450, T29H11.190, F11C18.12, F14M2.15, F14M2.14, F6A4_170, decreased number, F6F3_8, P450, F14M2_14, F14M2_15, CYP450 Superfamily, Cytochrome P 450 Enzyme, K15C23_6, CONSTITUTIVE PHOTOMORPHOGENIC DWARF, F6F3.25, F21J6_102, T4C9_170, FCAALL.280, P 450 Enzymes, CYP450, FCAALL.277, T20P8_6, KLUH, T20P8_5, F6G17.1, F6G17.6, Cytochrome P-450-Dependent Monooxygenase, F6G17.5, F6G17.8, family 90, Enzymes, T3A4_4, CYP90A1, family 93, Micro RNA, family 91, F6G17.10, family 98, family 97, F1C12.160B, F1C12.160A, F6G17_10, F11F12_12, T29A15.200, family 83, family 82, family 81, Cytochrome P-450 Monooxygenase, family 87, DL3725W, family 86, T4C9_150, family 89, family 88, F1C12.160, Primary MicroRNA, Cytochrome P 450 Superfamily, F6G17_20, F6G17.20, YUP8H12R_1, T21P5.11, F8N16.14, family 72, family 71, F8N16.15, ATKAO1, T4C9_160, family 76, Cytochrome P-450-Dependent, family 79, family 78, family 77, F11F12.12, F11F12.13, F8N16_14, F8N16_15, T12H17.100, Cytochrome P 450 Families, K8K14.3, F11F12_13, T21P5_11falseHomo sapiensRelationship between Differential Hepatic microRNA Expression and Decreased Hepatic Cytochrome P450 3A Activity in Cirrhosis2025-09-242014-02-11PRJNA212633GSE49012960624058572