ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR178/004/SRR1787814/SRR1787814_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR178/004/SRR1787804/SRR1787804.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR178/006/SRR1787816/SRR1787816_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR178/000/SRR1787810/SRR1787810.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR178/008/SRR1787808/SRR1787808.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR178/005/SRR1787815/SRR1787815.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR178/009/SRR1787809/SRR1787809.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR178/002/SRR1787812/SRR1787812.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR178/006/SRR1787806/SRR1787806.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR178/005/SRR1787805/SRR1787805.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR178/007/SRR1787807/SRR1787807.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR178/001/SRR1787811/SRR1787811.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR178/003/SRR1787803/SRR1787803.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR178/004/SRR1787814/SRR1787814_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR178/003/SRR1787813/SRR1787813.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR178/006/SRR1787816/SRR1787816_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR178/002/SRR1787802/SRR1787802.fastq.gzprimaryOK200GenomicsJeremy Boss, Microbiology and Immunology, Emory Universityhttps://www.ebi.ac.uk/ena/browser/view/PRJNA230503Homo sapiensThe MHC class II transactivator (CIITA) is essential for the expression of MHC-II genes. CIITA functions as a transcriptional activator when bound to genes although repressive roles have been reported at some loci in other cell types. To define the role of CIITA in gene regulation in human B cells, we performed ChIP-seq for CIITA using three independent antibodies, the histone marks H3K4me3 and H3K27ac, and ATAC-seq in the Raji human B cell line. Additionally, we profiled H3K27ac and ATAC-seq in the CIITA-null RJ2.2.5 cell line. A core set of CIITA peaks were identified that overlapped in all antibodies. Using the H3K27ac, H3K4me3, and ATAC-seq profiles generated in CIITA+ Raji and CIITA-null RJ2.2.5 cells CIITA dependent chromatin modifications were identified. These data define the CIITA regulated genes in human B cells. Overall design: Examination of CIITA binding sites and correlation with active histone modifiations and accessible chromatin in Raji and RJ2.2.5 cellsxref:PubMed:25753668ENACIITA, CIITAIV, B-cell, B Cells, Materials, human being, Genetic, Man (Taxonomy), NLRA, B Lymphocytes, Modern, Gene, Bursa-Equivalent Lymphocyte, Mhc2ta, Cistrons, man, human, Bursa-Dependent Lymphocytes., Human, 2.3.1.-, C2ta, C2TA, 2.7.11.1, EG669998, B lymphocyte, Homo sapiens, Material, B-Lymphocyte, MHC2TA, Modern Man, Genetic Materials, Cistron, B-lymphocyte, Man, Genetic Material, Gm9475Human, Modern., human being, Man (Taxonomy), Homo sapiens, man, Man, human, Modern ManfalseHomo sapiensCIITA regulated genes in human B cells2025-09-242015-03-08PRJNA230503GSE52941960625753668