ENAGenomicsLaboratory of RNA Function, Graduate School of Medicine, Osaka Universityhttps://www.ebi.ac.uk/ena/browser/view/PRJNA231072Homo sapiensxref:PubMed:24954906We recently identified the RNA targets of Ataxin-2 by using PAR-CLIP. To elucidate the functional role of Ataxin-2 on target-binding, we have knocked down or overexpressed Ataxin-2 in HEK293T cells and extracted RNAs 48 hrs after transfection. Then, these total RNAs were subjected to whole transcripts microarray analysis to examine how the perturbation of Ataxin-2 expression affects target gene expression. Overall design: Ataxin-2 was either knocked down using siRNA or overexpressed using the expression construct of Halo-Ataxin-2.Control siRNA was transfected as a control of knockdown experiment. The expression construct of Halo-tag alone was used as a control of overexpression experiment. Forty-eight hrs after transfection, total RNAs were extracted and subjected to microarray analysis.ENAAtx-2, Ataxin-2, SCA2, Materials, Genetic, determination, Material, Spinocerebellar Ataxia Type 2 Protein, Atx 2 Protein, SCA2 Protein, chemical analysis, Protein, Ataxin 2 Protein, Ataxin 2, ATXN2 Protein, Ataxin-2 Protein, ATXN2, Genetic Materials, Cistron, Gene, assay, Genetic Material., Cistrons, Atx-2 ProteinHuman, Modern., human being, Man (Taxonomy), Homo sapiens, man, Man, human, Modern ManfalseHomo sapiensAnalysis of the expression of Ataxin-2-target genes2025-09-242014-06-22PRJNA231072GSE53189960624954906