ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR112/009/SRR1122229/SRR1122229.fastq.gzprimaryOK200GenomicsHT1080SBMRIhttps://www.ebi.ac.uk/ena/browser/view/PRJNA233511Homo sapiensWe expressed Mycoplasma hyorhinis MTases MhyI and MhyII in cultured human cells. To determine the genome-wide effect of MhyI and MhyII-induced methylation, we performed global methylation profiling of the genomic DNA isolated from HT1080/MhyI, HT1080/MhyII and HT1080/mock cells. We employed the genome-wide Methylated DNA ImmunoPrecipitation – genome sequencing (MeDIP-seq). To obtain the 5mC-enriched DNA fraction, we used an anti-5mC antibody (clone 10G4) that is highly specific to 5mC in both the CG- and GATC-methylated sequences. We sequenced DNAs in the MeDIP fraction and recorded over 10 Gb of sequence reads per sample.ENAHuman, strain, human being, Man (Taxonomy), Homo sapiens, Modern Man, Modern, Sprain, Strain., cultivar, Strains, Sprains, Strains and Sprains, man, Man, ecotype, humanthymus nucleic acid, human being, Man (Taxonomy), DNS, (Deoxyribonucleotide)n, DNAn+1, Modern, Double Stranded, Deoxyribonucleic acid, Double-Stranded, man, human, Cell, Deoxyribonucleic acids, Human, (Deoxyribonucleotide)n+m, Homo sapiens, Deoxyribonucleic Acid, Modern Man, ds DNA, Desoxyribonukleinsaeure, Double-Stranded DNA, (Deoxyribonucleotide)m, DNA, deoxyribonucleic acids, ds-DNA, DNAn, desoxyribose nucleic acid, Man, Methyltransferase.falseHomo sapiens strain:HT1080Methylome in human cells expressing mycoplasmal DNA methyltransferases2025-09-242014-01-12PRJNA2335119606