ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR163/002/SRR1630892/SRR1630892.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR110/003/SRR1104823/SRR1104823.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR163/004/SRR1630894/SRR1630894.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR163/000/SRR1630900/SRR1630900.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR163/000/SRR1630890/SRR1630890.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR163/008/SRR1630888/SRR1630888.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR163/009/SRR1630899/SRR1630899.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR110/008/SRR1104828/SRR1104828.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR163/008/SRR1630898/SRR1630898.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR163/006/SRR1630896/SRR1630896.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR110/007/SRR1104827/SRR1104827.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR110/005/SRR1104825/SRR1104825.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR163/001/SRR1630901/SRR1630901.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR163/003/SRR1630893/SRR1630893.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR163/009/SRR1630889/SRR1630889.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR163/005/SRR1630895/SRR1630895.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR110/004/SRR1104824/SRR1104824.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR163/001/SRR1630891/SRR1630891.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR110/006/SRR1104826/SRR1104826.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR163/007/SRR1630897/SRR1630897.fastq.gzprimaryOK200GenomicsHannon's, Cold Spring Harborhttps://www.ebi.ac.uk/ena/browser/view/PRJNA233963Mus musculusIn mammals, piRNA populations are dynamic throughout male germ cell development. Embryonic piRNAs consist of both primary and secondary species and are mainly directed toward transposons. In meiotic cells, however, the piRNA population is transposon-poor and restricted to primary piRNAs derived from pachytene piRNA clusters. The mechanism controlling which piRNAs are present at each developmental stage is poorly understood. Here we show that RNF17 shapes adult meiotic piRNA content by suppressing the production of secondary piRNAs. In the absence of RNF17, ping-pong (secondary amplification) occurs inappropriately in meiotic cells – aberrantly targeting protein-coding genes and lncRNAs. Our data indicate that RNF17 comprises one component of a “refereeing” mechanism that prevents deleterious activity of the meiotic piRNA pathway by ensuring the selective loading of PIWI proteins with products of meiotic piRNA clusters. Overall design: Examination of small RNA profile in heterozygous and homozygous RNF17 adult testes, pachytene or round spermatid sorted cellsENAMus musculus, Laboratory Mice., House, Mus, Laboratory, Swiss, Mus domesticus, mouse, Mus musculus domesticus, Swiss Mouse, mouse <Mus musculus>, Mouse, House Mice, Swiss Mice, house mouse, Mice, Laboratory Mouse, House Mouse, mice C57BL/6xCBA/CaJ hybrid, domesticus, Mus muscarisMus musculus, Laboratory, testicle, Swiss, Testicle, mice, Mus domesticus, mouse, Swiss Mouse, male gonad, House Mice, Swiss Mice, SPATA23, Mmip-2, Mmip2, House Mouse, mice C57BL/6xCBA/CaJ hybrid, domesticus, Mus muscaris, Laboratory Mice, gonad of male reproductive system, MMIP-2, House, Mus, orchis, TDRD4, gonad of male genitalia., Testicles, Mus musculus domesticus, Mouse, house mouse, testiculus, testes, Mice, Laboratory Mouse, TestesfalseMus musculusRNF17 referees ping-pong in mouse testes [smallRNAseq]2025-09-242014-12-17PRJNA233963GSE5391710090