{"database":"ENA","file_versions":[],"scores":null,"additional":{"omics_type":["Genomics"],"center_name":["Computational Biology and Bionformatics, Cell and Developmental Biology, Max Planck Institute for Molecular Biomedicine"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJNA238542"],"scientific_name":["Homo sapiens"],"long_description":["We used collection of sequentially mutated BM-hMSCs ranging from wild type (no oncogenic hits) to fully transformed hMSCs (targeted with up six oncogenic mutations)to address whether BM-hMSCs at different stages of a well-characterized oncogenic process (normal, immortalized, transformed) retain immunomodulatory properties in vitro and in vivo. We characterize, for the first time, an oncogenic transformation-associated loss of the immunesuppressive and anti-inflammatory properties by hMSCs and identify candidate immune effectors underlying the loss of immunomodulation in transformed hMSCs. Overall design: Wild type BM-hMSCs (MSC-0H-GFP) did not develop tumors, meanwhile hMSCs transformed sequentially transformed with 5 oncogenic lesions (p53 inactivation Rb inactivation expression of hTERT stabilization of c-myc and expression of oncogenic H-RAS) (MSC-5H-GFP) cells gave rise to undifferentiated sarcomas when inoculated into immunedeficient mice. The tumoral transformation process causes the loss of the immunosuppressive and anti-inflamatory propierties of MSC-5H-GFP cells as compared to those of the wild type MSC-0H-GFP cells. To understand the basis of this loss of immunosuppression in transformed hMSCs, gene expression analysis was performed to create the list of differentially expressed genes between MSC-0H-GFP and MSC-5H-GFP cells. Further analysis of altered signaling pathways and upstream regulators were performed using the Ingenuity Pathway Analysis software."],"tag":["xref:PubMed:25358789"],"repository":["ENA"],"additional_accession":[]},"is_claimable":false,"name":"Homo sapiens","description":"Loss of immunosuppressive and anti-inflammatory properties in transformed human mesenchymal stem cells","dates":{"last_updated":"2025-09-24","first_public":"2015-05-20"},"accession":"PRJNA238542","cross_references":{"GEO":["GSE55108"],"taxon":["9606"],"PubMed":["25358789"]}}