ENAGenomicsBeiting, Pathobiology, University of Pennsylvaniahttps://www.ebi.ac.uk/ena/browser/view/PRJNA240325Homo sapiensxref:PubMed:25036052xref:PubMed:28192528xref:PubMed:29367210The host immune response plays a critical role not only in protection from human leishmaniasis, but also in promoting disease severity. Although candidate gene approaches in mouse models of leishmaniasis have been extremely informative, a global understanding of the immune pathways active in lesions from human patients is lacking. To address this issue, genome-wide transcriptional profiling of Leishmania braziliensis-infected cutaneous lesions and normal skin controls was carried out. A signature of the L. braziliensis skin lesion was defined that includes over 2,000 differentially regulated genes. Pathway-level analysis of this transcriptional response revealed key biological pathways, as well as specific genes, associated with cutaneous pathology, generating a testable 'metapathway' model of immune-driven lesion pathology, and providing new insights for treatment of human leishmaniasis. Overall design: Thirty-five skin biopsies were analyzed, including 10 normal skin biopsies (2 from North America and 8 from non-endemic area in Brazil), and 25 skin lesion biopsies (8 early cutaneous lesions, 17 late cutaneous lesions) obtained from Leishmania brazilensis-infected patients presenting at the Corte de Pedra Health Post in Corte de Pedra, Bahia, Brazil.ENAHuman, Leishmania (Viannia) braziliensis, Leishmania brasiliensis brasiliensis, human being, Leishmania viannia braziliensis, Leishmania brasiliensis peruviana, Leishmania braziliensis peruviana, Man (Taxonomy), Leishmania brasiliensis, Homo sapiens, Leishmania viannia, Modern Man, Leishmania braziliensis braziliensis., Modern, Leishmania (Viannia) brasiliensis, Leishmania viannia brasiliensis, Man, human, humans, Leishmania (V.) braziliensisHuman, Modern., human being, Man (Taxonomy), Homo sapiens, man, Man, human, Modern ManfalseHomo sapiensGenomic profiling of human Leishmania braziliensis lesions identifies transcriptional modules associated with cutaneous immunopathology2025-09-242014-03-08PRJNA240325GSE556649606293672102819252825036052