{"database":"ENA","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Fastqsanger.gz":["ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR126/000/SRR1265700/SRR1265700.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR126/001/SRR1265701/SRR1265701.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR126/002/SRR1265702/SRR1265702.fastq.gz"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Genomics"],"center_name":["OHSU"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJNA245819"],"scientific_name":["Homo sapiens"],"long_description":["RNA interference is an important component of innate antiviral defense in invertebrates and facilitated through production of viral-derived short interfering RNAs (siRNAs). There remains lack of evidence for siRNA production in vertebrate cells--specifically, mammalian somatic cells however, these cells do express cellular microRNAs (miRNAs) with inhibitory function. Additionally, a number of DNA viruses express viral miRNAs with roles in the viral life cycles. Flaviviruses, such as Dengue virus (DENV4) or West Nile virus (WNV), can infect both invertebrate and mammalian cells. To examine whether these viruses express either siRNAs or viral miRNAs in infected mammalian cells, we analyzed Huh7 cells infected with DENV subtype 4 (DENV4) or with WNV strain TX-IC7. We identified a large number of cellular miRNAs however, only 1% of the small RNAs were of viral origin and none of them exhibited characteristics of viral miRNAs or siRNAs."],"tag":["xref:EuropePMC:PMC4097787"],"repository":["ENA"],"additional_accession":[]},"is_claimable":false,"name":"Homo sapiens","description":"Small RNA sequencing of flavivirus-infected Huh7 cells","dates":{"last_updated":"2025-09-24","first_public":"2014-05-10"},"accession":"PRJNA245819","cross_references":{"taxon":["9606"]}}