{"database":"ENA","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Fastqsanger.gz":["ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR134/009/SRR1342219/SRR1342219_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR134/006/SRR1342216/SRR1342216_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR134/007/SRR1342217/SRR1342217_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR134/008/SRR1342218/SRR1342218_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR134/009/SRR1342219/SRR1342219_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR134/008/SRR1342218/SRR1342218_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR134/007/SRR1342217/SRR1342217_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR134/006/SRR1342216/SRR1342216_2.fastq.gz"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Genomics"],"center_name":["Animal Sciences and Health, Institute of Biology, Leiden University"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJNA251728"],"scientific_name":["Danio rerio"],"tag":["xref:PubMed:25086293"],"long_description":["Scavenger receptors on the cell surface of macrophages play an important role in host defence through their ability to bind microbial ligands and induce phagocytosis. Concurrently, signal transduction pathways are initiated that aid in defence mechanisms against the invading microbe. Here we report on the function of scavenger receptor Marco (macrophage receptor with collagenous structure) during infection of zebrafish embryos with Mycobacterium marinum, a close relative of Mycobacterium tuberculosis. Morpholino knockdown demonstrates that Marco is required for the rapid phagocytosis of M. marinum following intravenous infection. Furthermore, gene expression analysis shows that Marco controls the initial transient pro-inflammatory response to M. marinum and remains a determining factor for the immune response signature at later stages of infection. Increased bacterial burden following marco knockdown indicates that this scavenger receptor is important for control of M. marinum growth, likely due to delayed phagocytosis and reduced pro-inflammatory signalling observed under conditions of Marco deficiency Overall design: Embryos were injected at the one cell stage with a morpholino targeting marco, or with the standard control morpholino from GeneTools for comparison. Subsequently, at 24 hours post fertilization (hpf) the morphants and their controls were manually dechorionated at 24 hpf and at 28 hpf they were infected by injecting 200 colony forming units of M. marinum Mma20 into the caudal vein, or mock-injected with PBS/2%PVP. After injections embryos were transferred into fresh egg water containing 0.003% 1-phenyl-2-thiourea (Sigma-Aldrich) to prevent melanization and incubated for 4 days at 28°C. After the incubation period, infected and uninfected morphants, mutants and their controls were imaged and groups of 30 embryos were snap-frozen in liquid nitrogen and RNA was isolated for Illumina RNAseq analysis."],"repository":["ENA"],"name_synonyms":["zebra fish <Danio rerio>, zebra fish, Danio rerio (Hamilton, Zebra, leopard danio, Danio rerio, Zebra Fish, Danio frankei, Zebrafishes, Fishes, Cyprinus rerio Hamilton, danio, Brachydanio rerio frankei., Zebra danios, Zebra danio, zebra danio, B. rerio, Danio rerio frankei, 1822), Zebra Fishes, 1822, D. rerio, Brachydanio rerio, Cyprinus rerio, Brachidanio rerio, zebrafish, D. rerios"],"description_synonyms":["zebra fish, signalling., Danio rerio (Hamilton, Controlling, leopard danio, biological signaling, preventive therapy, Zebra Fish, Monocyte Derived Macrophages, Monocyte Derived, Cyprinus rerio Hamilton, prevention, Monocyte-Derived, Mycobacteria, zebra danio, Danio rerio frankei, 1822), Monocyte-Derived Macrophage, 1822, Brachydanio rerio, Cyprinus rerio, Brachidanio rerio, Phagocytoses, prevention and control, D. rerios, Monocyte-Derived Macrophages, Macrophage, Zebra, Danio rerio, reference sample, Danio frankei, Macrophages, Zebrafishes, prophylaxis, Bone Marrow Derived Macrophages, Fishes, Bone Marrow-Derived Macrophage, danio, Zebra danios, Bone Marrow-Derived, preventive measures, Zebra danio, signalling process, control, B. rerio, signaling process, Zebra Fishes, Bone Marrow-Derived Macrophages, D. rerio, zebrafish, Brachydanio rerio frankei, Controlled, single organism signaling"],"additional_accession":[]},"is_claimable":false,"name":"Danio rerio","description":"Phagocytosis of mycobacteria by zebrafish macrophages is dependent on the scavenger receptor Marco, a key control factor of pro-inflammatory signalling","dates":{"last_updated":"2025-09-24","first_public":"2014-08-08"},"accession":"PRJNA251728","cross_references":{"GEO":["GSE58230"],"taxon":["7955"],"PubMed":["25086293"]}}