{"database":"ENA","file_versions":[],"scores":null,"additional":{"omics_type":["Genomics"],"center_name":["Department of Microbiology and Immunology, University of California San Francisco"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJNA251899"],"scientific_name":["Mus musculus"],"long_description":["The generation of naïve T lymphocytes is critical for immune function yet the mechanisms governing their maturation remain incompletely understood. We have identified a mouse mutant, bloto, that harbors a hypomorphic mutation in the zinc finger protein Zfp335. Mutant blt/blt mice exhibit a naïve T cell deficiency due to an intrinsic developmental defect that begins to manifest in the thymus and continues into the periphery, affecting T cells that have recently undergone thymic egress. Zfp335 binds to promoter regions via a consensus motif, and its target genes are enriched in categories related to protein metabolism, mitochondrial function and transcriptional regulation. Restoring the expression of one target, Ankle2, partially rescues T cell maturation. Our findings identify Zfp335 as a transcription factor and essential regulator of late-stage intrathymic and post-thymic T cell maturation. Overall design: WT and blt/blt mature CD4SP thymocytes were sorted from mixed hematopoietic chimeras for RNA extraction and gene expression analysis on Affymetrix arrays. 6 samples were analyzed in total, with 3 biological replicates per genotype."],"tag":["xref:PubMed:25343476","xref:PubMed:27401554"],"repository":["ENA"],"name_synonyms":["Mus musculus, Laboratory Mice., House, Mus, Laboratory, Swiss, Mus domesticus, mouse, Mus musculus domesticus, Swiss Mouse, mouse <Mus musculus>, Mouse, House Mice, Swiss Mice, house mouse, Mice, Laboratory Mouse, House Mouse, mice C57BL/6xCBA/CaJ hybrid, domesticus, Mus muscaris"],"description_synonyms":["Aethylnitroso-harnstoff, Profiling, Nif1, Transcriptome, T-cell surface antigen T4/Leu-3, T-cell surface antigen T4|Leu-3, Laboratory, N-Ethyl-N-nitroso carbamide, Mus domesticus, Sprain, mouse, Monitorings, Gene, N-Ethyl-N-nitroso-urea, House Mouse, mice C57BL/6xCBA/CaJ hybrid, Mus muscaris, Gene Expression Profilings, strain, Gene Expression, Differential Display, Transcript Expression Analysis, transcription profiling, NIF-1, 1810045J01Rik., L3T4, mRNA Differential Displays, Thymocyte, Transcript Expression Analyses, Gene Expression Pattern Analysis, N-Ethylnitrosourea, T-cell differentiation antigen L3T4, House, Mus, Strain, Gene Expression Monitorings, Ly-4, Mus musculus domesticus, cultivar, Analysis, Profilings, mRNA Differential Display, Mice, Sprains, gene expression profiling, Mus musculus, Gene Expression Monitoring, Transcriptome Profiling, Analyses, mRNA, Swiss, Transcriptome Profilings, mice, ENU, T-cell surface glycoprotein CD4, Swiss Mouse, 1-Ethyl-1-nitrosourea, Znf335, Transcriptomics, CD4mut, House Mice, Swiss Mice, Differential Displays, Transcript Expression, Transcriptome Analysis, Transcriptome Analyses, ecotype, Monitoring, 1-(Aminocarbonyl)-1-ethyl-2-oxohydrazine, domesticus, Laboratory Mice, Gene Expression Analysis, Gene Expression Analyses, Nif, CD4, Ethyl nitrosourea, Expression Analysis, Mouse, Strains, house mouse, Expression Analyses, Strains and Sprains, Laboratory Mouse, NEU"],"additional_accession":[]},"is_claimable":false,"name":"Mus musculus","description":"Gene expression profiling of mature CD4 SP thymocytes from a mouse strain with an ENU-induced mutation in Zfp335","dates":{"last_updated":"2025-09-24","first_public":"2014-10-18"},"accession":"PRJNA251899","cross_references":{"GEO":["GSE58288"],"taxon":["10090"],"PubMed":["25343476","27401554"]}}