ENAGenomicsDepartment of Microbiology and Immunology, University of California San Franciscohttps://www.ebi.ac.uk/ena/browser/view/PRJNA251899Mus musculusThe generation of naïve T lymphocytes is critical for immune function yet the mechanisms governing their maturation remain incompletely understood. We have identified a mouse mutant, bloto, that harbors a hypomorphic mutation in the zinc finger protein Zfp335. Mutant blt/blt mice exhibit a naïve T cell deficiency due to an intrinsic developmental defect that begins to manifest in the thymus and continues into the periphery, affecting T cells that have recently undergone thymic egress. Zfp335 binds to promoter regions via a consensus motif, and its target genes are enriched in categories related to protein metabolism, mitochondrial function and transcriptional regulation. Restoring the expression of one target, Ankle2, partially rescues T cell maturation. Our findings identify Zfp335 as a transcription factor and essential regulator of late-stage intrathymic and post-thymic T cell maturation. Overall design: WT and blt/blt mature CD4SP thymocytes were sorted from mixed hematopoietic chimeras for RNA extraction and gene expression analysis on Affymetrix arrays. 6 samples were analyzed in total, with 3 biological replicates per genotype.xref:PubMed:25343476xref:PubMed:27401554ENAMus musculus, Laboratory Mice., House, Mus, Laboratory, Swiss, Mus domesticus, mouse, Mus musculus domesticus, Swiss Mouse, mouse <Mus musculus>, Mouse, House Mice, Swiss Mice, house mouse, Mice, Laboratory Mouse, House Mouse, mice C57BL/6xCBA/CaJ hybrid, domesticus, Mus muscarisAethylnitroso-harnstoff, Profiling, Nif1, Transcriptome, T-cell surface antigen T4/Leu-3, T-cell surface antigen T4|Leu-3, Laboratory, N-Ethyl-N-nitroso carbamide, Mus domesticus, Sprain, mouse, Monitorings, Gene, N-Ethyl-N-nitroso-urea, House Mouse, mice C57BL/6xCBA/CaJ hybrid, Mus muscaris, Gene Expression Profilings, strain, Gene Expression, Differential Display, Transcript Expression Analysis, transcription profiling, NIF-1, 1810045J01Rik., L3T4, mRNA Differential Displays, Thymocyte, Transcript Expression Analyses, Gene Expression Pattern Analysis, N-Ethylnitrosourea, T-cell differentiation antigen L3T4, House, Mus, Strain, Gene Expression Monitorings, Ly-4, Mus musculus domesticus, cultivar, Analysis, Profilings, mRNA Differential Display, Mice, Sprains, gene expression profiling, Mus musculus, Gene Expression Monitoring, Transcriptome Profiling, Analyses, mRNA, Swiss, Transcriptome Profilings, mice, ENU, T-cell surface glycoprotein CD4, Swiss Mouse, 1-Ethyl-1-nitrosourea, Znf335, Transcriptomics, CD4mut, House Mice, Swiss Mice, Differential Displays, Transcript Expression, Transcriptome Analysis, Transcriptome Analyses, ecotype, Monitoring, 1-(Aminocarbonyl)-1-ethyl-2-oxohydrazine, domesticus, Laboratory Mice, Gene Expression Analysis, Gene Expression Analyses, Nif, CD4, Ethyl nitrosourea, Expression Analysis, Mouse, Strains, house mouse, Expression Analyses, Strains and Sprains, Laboratory Mouse, NEUfalseMus musculusGene expression profiling of mature CD4 SP thymocytes from a mouse strain with an ENU-induced mutation in Zfp3352025-09-242014-10-18PRJNA251899GSE58288100902534347627401554