ENAGenomicsSun-Yat-Sen Memorial Hospitalhttps://www.ebi.ac.uk/ena/browser/view/PRJNA255113Homo sapiensxref:PubMed:26436920To examine the miRNA expression profile of the profibrogenic macrophages, we compared miRNA expression in untreated versus IL-4 or IL-13-treated macrophages using miRNA microarray.IL-4 and IL-13 treatment resulted in marked changes in 18 and 19 miRNAs in macrophages, respectively.(n=3, fold changes>2, P<0.05) Overall design: Total RNA from human primary macrophages (n=3) treated with or without 20 ng/ml IL-4 or 20ng/ml IL-13 for 48 hr was isolated with a mirVana miRNA Isolation kit (Ambion, Foster, CA). The miRNA expression profile of the samples were examined using miRCURY LNA™ arrays (v18.0 covering 1921 human miRNAs, Exiqon ,Vedbaek, Denmark).ENAfalseHomo sapiensM2 cytokines alter the miRNA expression profile in macrophages2025-09-242014-07-13PRJNA255113GSE59348960626436920