ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR140/062/SRR14083062/SRR14083062_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR140/059/SRR14083059/SRR14083059.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR140/060/SRR14083060/SRR14083060_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR140/059/SRR14083059/SRR14083059_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR140/061/SRR14083061/SRR14083061_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR140/060/SRR14083060/SRR14083060.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR140/064/SRR14083064/SRR14083064.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR140/058/SRR14083058/SRR14083058_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR140/062/SRR14083062/SRR14083062_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR140/058/SRR14083058/SRR14083058.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR140/062/SRR14083062/SRR14083062.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR140/061/SRR14083061/SRR14083061_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR140/057/SRR14083057/SRR14083057_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR140/061/SRR14083061/SRR14083061.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR140/063/SRR14083063/SRR14083063_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR140/059/SRR14083059/SRR14083059_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR140/064/SRR14083064/SRR14083064_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR140/060/SRR14083060/SRR14083060_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR140/057/SRR14083057/SRR14083057.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR140/063/SRR14083063/SRR14083063_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR140/064/SRR14083064/SRR14083064_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR140/063/SRR14083063/SRR14083063.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR140/058/SRR14083058/SRR14083058_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR140/057/SRR14083057/SRR14083057_1.fastq.gzprimaryOK200GenomicsCSIR-IGIBhttps://www.ebi.ac.uk/ena/browser/view/PRJNA258569Homo sapiensThe objective of this study was to identify and analyze somatic variations in healthy human tissues. For this we obtained paired tissue from nine individuals. Four tissue pairs represented neuronal and non-neuronal parts of the brain - frontal cortex (FC) and corpus callosum (CC), respectively. These were from post-mortem, road accident victims. The other five tissue pairs were from blood and saliva of unrelated healthy adults. Exome enrichment was performed on DNA isolated from these 18 samples using Illumina Tru-Seq chemistry and then sequenced on Hi-Seq2000 (100 base pair, paired-end). The sequence reads were filtered for quality and then aligned against the reference human genome (hg19) using BWA. We observed brain to harbor more somatic variations than other tissue types – and majority of them were transversions likely to have occurred due to oxidative stress.ENAthe brain, grade IV adult astrocytic tumour, Genomics, suprasegmental structures, human being, Comparative, Modern, Functional Genomics, Giant Cell, Grade IV, Astrocytomas, encephalon, Human, adult glioblastoma multiforme, Structural, Client., Homo sapiens, primary glioblastoma multiforme, Functional, tissue portion, glioblastoma, simple tissue, synganglion, Giant Cell Glioblastomas, Man, glioblastoma multiforme, portion of tissue, Glioblastoma, Man (Taxonomy), grade IV adult astrocytic tumor, spongioblastoma multiforme, Comparative Genomics, Encephalon, Tissue, Glioblastomas, GBM, suprasegmental levels of nervous system, Giant Cell Glioblastoma, man, human, Astrocytoma, Glioblastoma Multiforme, Grade IV Astrocytomas, Patient, grade IV adult Astrocytic tumor, Clients, Modern Man, Structural Genomics, Grade IV AstrocytomaHuman, Modern., human being, Man (Taxonomy), Homo sapiens, man, Man, human, Modern ManfalseHomo sapiensTissue specific genomics of human brain in healthy and glioblastoma patients2025-09-242015-08-25PRJNA2585699606