{"database":"ENA","file_versions":[],"scores":null,"additional":{"omics_type":["Genomics"],"center_name":["Pathology, University of California, San Diego"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJNA260312"],"scientific_name":["Mus musculus"],"long_description":["The diversity of human breast cancer subtypes has led to the hypothesis that breast cancer is a number of different diseases arising from cells at various stages of differentiation. We have derived clonal multipotent metastatic mammary cancer stem cells from the polyomavirus middle T mouse model of breast cancer, that can differentiate into luminal, myoepithelial and alveolar cells. When injected orthotopically at low-density, the resulting tumors express estrogen and progesterone receptors. With continued passage in vivo, the tumor cells undergo additional epigenetic and/or genetic changes that result in upregulation of Her2 expression or clonal expansion of cells that give rise to basal-like or claudin-low tumors. As in human tumors, the more aggressive tumor subtypes express nuclear p53. The temporal sequence of events suggests that contrary to current dogma, multiple tumor subtypes can originate from a single multipotent cancer stem cell that undergoes evolution during tumor progression. Furthermore, these data raise the possibility that a human polyomavirus may be a causative agent in spontaneous forms of human breast cancer. Overall design: Three spontaneous PyVmT tumors and forty cell line derived tumors were analyzed. To generate cell line derived tumors, 10^6 tumor cells were injected orthotopically into four abdominal mammary fat pads in syngeneic C57Bl/6 female mice. After 6-8 weeks the mice were euthanized and the tumors excised and frozen at -80°C. One tumor per mouse 8-10mm in diameter was used for array analysis. RNA was extracted using the RNeasy kit (Qiagen) with on column DNA removal."],"tag":["xref:PubMed:26467658"],"repository":["ENA"],"description_synonyms":["Breast Carcinoma, Human Mammary Neoplasm, primary breast cancer, Carcinoma, malignant tumor of the breast, Colony Forming Unit, Breast Carcinomas, \"breast neoplasm\" EXACT [MTH:120], Malignant Neoplasm, Neoplasms, Progenitor Cells, Colony-Forming Units, Progenitor Cell, Benign Neoplasm, mammary neoplasm, Mammary Cancers, Human Mammary Neoplasms, Tumor, \"breast tumor\" EXACT [NCI2004_11_17:C2910], Malignant, Cell, Human, Breast Malignant Neoplasm, Breast Malignant Tumor, Benign, Colony-Forming Unit, Stem Cell, \"neoplasm of breast (disorder)\" EXACT [SNOMEDCT_2005_07_31:126926005], Mammary Carcinomas, Breast Tumor, Neoplasm, Mother Cells, Human Mammary Carcinoma, \"mammary tumor\" EXACT [CSP2005:2016-0671], breast tumor, Malignant Neoplasm of Breast, Progenitor, Carcinomas, Mother Cell, Mammary Neoplasms, malignant neoplasm of breast, Human Mammary, Breast Neoplasm, Malignancy, Mammary Neoplasm, Stem, Mammary Carcinoma, Human Mammary Carcinomas, Mother, stem cell, Benign Neoplasms, Colony Forming Units, Cancers, \"mammary neoplasm\" RELATED [], Cancer of the Breast, animal stem cell, Neoplasias, Malignant Tumor of Breast, Mammary, Mammary Cancer, Breast Tumors, Breast Cancer, Cells, Breast Malignant Tumors, Cancer of Breast, Malignancies, Breast, mammary cancer, other neoplasm, mammary tumor, Breast Malignant Neoplasms, Neoplasia, Tumors, Cancer, Malignant Neoplasms."],"name_synonyms":["Mus musculus, Laboratory Mice., House, Mus, Laboratory, Swiss, Mus domesticus, mouse, Mus musculus domesticus, Swiss Mouse, mouse <Mus musculus>, Mouse, House Mice, Swiss Mice, house mouse, Mice, Laboratory Mouse, House Mouse, mice C57BL/6xCBA/CaJ hybrid, domesticus, Mus muscaris"],"additional_accession":[]},"is_claimable":false,"name":"Mus musculus","description":"Multipotent mammary cancer stem cells integrate stem cell and clonal expansion theories of tumor progression","dates":{"last_updated":"2025-09-24","first_public":"2014-09-12"},"accession":"PRJNA260312","cross_references":{"GEO":["GSE61138"],"taxon":["10090"],"PubMed":["26467658"]}}