{"database":"ENA","file_versions":[],"scores":null,"additional":{"omics_type":["Genomics"],"center_name":["Institute of Immunity & Transplantation, University College London"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJNA263199"],"scientific_name":["Mus musculus"],"long_description":["This study set out to examine CD4 T cell differentiation in a mouse model of diabetes based on transgenic expression of ovalbumin under the control of the rat insulin promoter and co-expression of the DO11.10 transgene (DO11 x rip-mOVA mice). The transcriptome of T cells isolated from the pancreatic lymph nodes (lymph nodes draining the site of self antigen expression) was compared with that of T cells isolated from inguinal lymph nodes (non-draining lymph nodes). T cells were sorted based on expression of CD4, DO11.10 TCR (KJ-126), CD25 and CD69. Overall design: Primary cells from 6 week old DO11 x rip-mOVA mice were isolated ex-vivo from the pancreatic lymph nodes or inguinal lymph nodes. Cells were sorted by flow cytometry using antibodies to CD4, DO11.10 TCR (KJ-126+), CD25 and CD69. 3-6 replicates were collected per experimental group with each replicate deriving from 14 mice. RNA was isolated using the RNeasy micro kit (Qiagen)."],"tag":["xref:PubMed:25485678"],"repository":["ENA"],"additional_accession":[]},"is_claimable":false,"name":"Mus musculus","description":"Follicular helper T cell signature in Type 1 Diabetes","dates":{"last_updated":"2025-09-24","first_public":"2014-10-08"},"accession":"PRJNA263199","cross_references":{"GEO":["GSE62104"],"taxon":["10090"],"PubMed":["25485678"]}}