ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR161/009/SRR1611959/SRR1611959.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR161/003/SRR1611963/SRR1611963.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR161/002/SRR1611962/SRR1611962.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR161/005/SRR1611965/SRR1611965.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR161/001/SRR1611961/SRR1611961.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR161/006/SRR1611966/SRR1611966.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR161/008/SRR1611968/SRR1611968.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR161/007/SRR1611967/SRR1611967.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR161/000/SRR1611960/SRR1611960.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR161/004/SRR1611964/SRR1611964.fastq.gzprimaryOK200GenomicsGUDMAP Database Group, IGMM MRC Human Genetics Unithttps://www.ebi.ac.uk/ena/browser/view/PRJNA263812Mus musculusWe will use micro-dissection and FACS techniques to isolate cell types from the developing lower urogenital region and perform a transcriptional analysis using RNA-SEQ. This analysis will determine the transcriptional profile of each cell type, identify compartment specific transcripts, compartment specific transcript isoforms and cell-type specific long-noncoding RNAs. In addition the unbiased nature of RNA-SEQ will potentially identify novel transcripts that have not been annotated in the database. Overall design: Total RNA was obtained from FACS isolated embryonic day 17.5 bladders using Tg(Upk2-cre)6Xrw x Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo transgenic mice. The long term goal is to generate a transcriptional atlas of developing lower urogenital region.ENAfalseMus musculusRNA-SEQ analysis of the developing lower urogenital region2025-09-242014-12-10PRJNA263812GSE6231010090