{"database":"ENA","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Fastqsanger.gz":["ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR180/005/SRR1802085/SRR1802085.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR180/004/SRR1802084/SRR1802084.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR180/000/SRR1802080/SRR1802080.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR180/001/SRR1802081/SRR1802081.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR180/008/SRR1802078/SRR1802078.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR180/009/SRR1802079/SRR1802079.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR180/003/SRR1802083/SRR1802083.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR180/002/SRR1802082/SRR1802082.fastq.gz"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Genomics"],"center_name":["Department of General, Visceral and Pediatric Surgery, University Medical Center Göttingen"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJNA275306"],"scientific_name":["Homo sapiens"],"tag":["xref:PubMed:25788266"],"long_description":["Hormone-dependent gene expression requires dynamic and coordinated epigenetic changes. Estrogen receptor-positive (ER+) breast cancer is particularly dependent upon extensive chromatin remodeling and changes in histone modifications for the induction of hormone-responsive gene expression. Our previous studies established an important role of bromodomain-containing protein-4 (BRD4) in promoting estrogen-regulated transcription and proliferation of ER+ breast cancer cells. Here, we investigated the association between genome-wide occupancy of histone H4 acetylation at lysine 12 (H4K12ac) and BRD4 in the context of estrogen-induced transcription. Similar to BRD4, we observed that H4K12ac occupancy increases near the transcription start sites (TSS) of estrogen-induced genes as well as at distal ERα binding sites in an estrogen-dependent manner. Interestingly, H4K12ac occupancy highly correlates with BRD4 binding and enhancer RNA production on ERα-positive enhancers. Consistent with an importance in estrogen-induced gene transcription, H4K12ac occupancy globally increased in ER-positive cells relative to ER-negative cells and these levels were further increased by estrogen treatment in an ERα-dependent manner. Together, these findings reveal a strong correlation between H4K12ac and BRD4 occupancy with estrogen-dependent gene transcription and further suggest that modulators of H4K12ac and BRD4 may serve as new therapeutic targets for hormone-dependent cancers. Overall design: ChIP-seq profiles of H4K12ac in MCF7 cells treated with +/- estrogen treatment and MCF10A cells."],"repository":["ENA"],"additional_accession":[]},"is_claimable":false,"name":"Homo sapiens","description":"H4K12ac is regulated by estrogen receptor-alpha and is associated with BRD4 function and inducible transcription","dates":{"last_updated":"2025-09-24","first_public":"2015-05-04"},"accession":"PRJNA275306","cross_references":{"GEO":["GSE65886"],"taxon":["9606"],"PubMed":["25788266"]}}