ENAGenomicsUniversity of tsukubahttps://www.ebi.ac.uk/ena/browser/view/PRJNA278357Mus musculus【Objectives】The FPR2 is a G-protein coupled receptor that potently possesses pro- and anti-inflammatory role. Although FPR2 is known to be expressed by T cells, its function in arthritic condition is unclear. We investigated the involvement of FPR2 on T cells in GPI-induced arthritis (GIA) and rheumatoid arthritis (RA). 【Methods】Fluctuated expression of FPR2 mRNA on CD4+T cells was analyzed in GIA. We sorted FPR2+ or FPR2-CD4+T cells from arthritic lymph nodes, then the mRNA expression of various markers from CD4+T cell subsets was examined. Naïve T cells were cultured favoring Th1, Th2 and Th17 cell differentiation, then the expression of FPR2 was analyzed by FACS. In humans, the expression of FPR2 on CD4+T cells from healthy subjects (HS) or patients with RA was compared. 【Results】The expression of FPR2 in CD4+T cells was upregulated in the early phase of arthritis. FPR2+ T cells had higher expression of T-bet and IFNγ than FPR2-T cells. FPR2+ T cells were frequently detected on Th1 condition but not on Th2 and Th17. The expression of FPR2 on CD4+T cells was significantly higher in RA than in HS. 【Conclusion】We identified that FPR2+T cells showed Th1 phenotype in mice and this molecule was highly detected on CD4+T cells in patients with RA, suggesting FPR2+T cells might play a crucial role in RA. Overall design: To identify the highly expressed molecules on autoreactive-CD4+T cells in GPI-induced arthritis, the mRNA expression profile of splenic CD4+ T cells isolated by Magnetic–activated cell sorting (MACS) was examined by microarray in DBA/1 mice (arthritis susceptible) and C57BL/6 mice (arthritis resistant) after immunization of GPI on day7ENALy-4, CD4, Cell., L3T4, assay, CD4mut, T-cell surface antigen T4/Leu-3, determination, T-cell differentiation antigen L3T4, T-cell surface antigen T4|Leu-3, chemical analysis, T-cell surface glycoprotein CD4Mus musculus, Laboratory Mice., House, Mus, Laboratory, Swiss, Mus domesticus, mouse, Mus musculus domesticus, Swiss Mouse, mouse <Mus musculus>, Mouse, House Mice, Swiss Mice, house mouse, Mice, Laboratory Mouse, House Mouse, mice C57BL/6xCBA/CaJ hybrid, domesticus, Mus muscarisfalseMus musculusGenechip analysis of CD4+T cells2025-09-242015-03-17PRJNA278357GSE6691210090