ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR191/001/SRR1917141/SRR1917141.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR191/002/SRR1917122/SRR1917122.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR191/005/SRR1917135/SRR1917135.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR191/009/SRR1917139/SRR1917139.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR191/001/SRR1917131/SRR1917131.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR191/000/SRR1917150/SRR1917150.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR191/005/SRR1917125/SRR1917125.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR191/004/SRR1917144/SRR1917144.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR191/006/SRR1917136/SRR1917136.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR191/008/SRR1917138/SRR1917138.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR191/002/SRR1917142/SRR1917142.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR191/003/SRR1917123/SRR1917123.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR191/006/SRR1917126/SRR1917126.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR191/005/SRR1917145/SRR1917145.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR191/009/SRR1917129/SRR1917129.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR191/002/SRR1917132/SRR1917132.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR191/008/SRR1917148/SRR1917148.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR191/004/SRR1917124/SRR1917124.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR191/000/SRR1917130/SRR1917130.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR191/006/SRR1917146/SRR1917146.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR191/007/SRR1917127/SRR1917127.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR191/008/SRR1917128/SRR1917128.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR191/003/SRR1917133/SRR1917133.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR191/009/SRR1917149/SRR1917149.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR191/000/SRR1917140/SRR1917140.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR191/007/SRR1917147/SRR1917147.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR191/004/SRR1917134/SRR1917134.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR191/003/SRR1917143/SRR1917143.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR191/007/SRR1917137/SRR1917137.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR191/001/SRR1917121/SRR1917121.fastq.gzprimaryOK200GenomicsClaudia Bossen, Center for Chronic Immunodeficiency, University Hospital Freiburg (D)https://www.ebi.ac.uk/ena/browser/view/PRJNA278629Mus musculusxref:PubMed:25985234Early B cell development is orchestrated by the combined activities of the transcriptional regulators E2A, EBF1, Foxo1 and Ikaros. However, how the genome-wide binding patterns of these regulators are modulated during B-lineage development remains to be determined. Here, we found that in lymphoid progenitors the chromatin remodeler Brg1 specified the B cell fate. In committed pro-B cells Brg1 regulated Igh locus contraction and controlled c-Myc expression to modulate the expression of genes that regulate ribosome biogenesis. In committed pro-B cells Brg1 also suppressed a pre-B lineage-specific pattern of gene expression. Finally, we found that Brg1 acted mechanistically to establish B cell fate and modulate cell growth by facilitating access of lineage-specific transcription factors to poised enhancer repertoires. Overall design: 8 ATAC-Seq samples from sorted ALP and BLP (duplicates, control and Brg1-deleted), 4 ATAC-Seq samples from cultured pro-B cells (duplicates, control and Brg1-deleted), 2 Ikaros ChIP-seq samples (performed in Rag1-/- pro-B cells and in E2A-/- pre-pro-B cells), 1 Brg1 ChIP-seq sample and accompanying Input sample (both in Rag1-/- pro-B cells), 4 RNA-Seq samples from cultured pro-B cells (duplicates, control and Brg1-deleted), 6 RNA-Seq samples from cultured Rag1-/- pro-B cells (triplicates, control and Brg1-knock down).ENABRG1, Brg1, B-cell, B Cells, MRD16, SWI2, B Lymphocytes, Bursa-Dependent Lymphocytes, non-developmental growth of a unicellular organism, B cell, snf2, snf2b, brg1, BAF190, snf2l4, Bursa-Equivalent Lymphocyte, metabolic process resulting in cell growth, BAF190A, b2b508.1Clo, b2b692Clo, SW1|SNF, X-brg1, SNF2, baf190, SNF2L4, snf2-beta, cell expansion, SPAC29A4.21, RTPS2, snf2lb, B lymphocyte, SW1/SNF, B-Lymphocyte, snf2l, non-developmental cell growth, SNF2LB, HP1-BP72, rtps2, B-lymphocyte, metabolism resulting in cell growth., swi2, hSNF2b, SNF2betaMus musculus, Laboratory Mice., House, Mus, Laboratory, Swiss, Mus domesticus, mouse, Mus musculus domesticus, Swiss Mouse, mouse <Mus musculus>, Mouse, House Mice, Swiss Mice, house mouse, Mice, Laboratory Mouse, House Mouse, mice C57BL/6xCBA/CaJ hybrid, domesticus, Mus muscarisfalseMus musculusBrg1 activates enhancer repertoires to establish B cell identity and modulate cell growth2025-09-242015-05-15PRJNA278629GSE669781009025985234