{"database":"ENA","file_versions":[],"scores":null,"additional":{"omics_type":["Genomics"],"center_name":["Blizard Institute, Queen Mary, University of London"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJNA300869"],"scientific_name":["Homo sapiens"],"long_description":["Cancer stem cells (CSCs) drive tumour spread and therapeutic resistance, and can undergo epithelial-to-mesenchymal transition (EMT) and mesenchymal-to-epithelial transition (MET) to switch between epithelial and post-EMT sub-populations. Examining oral squamous cell carcinoma (OSCC), we now show that increased phenotypic plasticity, the ability to undergo EMT/MET, underlies increased CSC therapeutic resistance within both the epithelial and post-EMT sub-populations. The post-EMT CSCs that possess plasticity exhibit particularly enhanced therapeutic resistance and are defined by a CD44highEpCAMlow/-CD24+ cell surface marker profile. Treatment with TGFβ and retinoic acid (RA) enabled enrichment of this sub-population for therapeutic testing, through which the endoplasmic reticulum (ER) stressor and autophagy inhibitor Thapsigargin was shown to selectively target these cells. Demonstration of the link between phenotypic plasticity and therapeutic resistance, and development of an in vitro method for enrichment of a highly resistant CSC sub-population, provides an opportunity for the development of improved chemotherapeutic agents that can eliminate CSCs. Overall design: The CA1 OSCC cell line was sub-cloned to derive 4 clonal sub-lines, termed pEMT-P, pEMT-S, Epi-S and Epi-P (here 18, 23, 7 and 4 respectively)."],"tag":["xref:PubMed:26981578"],"repository":["ENA"],"description_synonyms":["Initiating Cell, OCSC, Carcinoma, Squamous Cell Carcinoma of the Mouth, Squamous Cell Carcinoma of the Nasal Cavity, Cancer Stem Cell, Unit, Colony-Forming Units, Stem Cells, Squamous Cell Carcinoma of the Head and Neck, Tumor Stem Cells, Head And Neck, Tumor, Squamous Cell Carcinoma, Cancer Stem, Tumor Stem, Cell, Neoplastic Stem, Hypopharyngeal Squamous Cell Carcinoma, Colony-Forming Unit, Stem Cell, Neoplastic Colony Forming Units, oral squamous cell carcinoma, Units, resistance, Oral Squamous Cell Carcinoma, Initiating Cells, HNSCC, Neoplastic, Oropharyngeal Squamous Cell Carcinoma, Neoplastic Colony-Forming Units, Tumor Initiating Cells, Laryngeal Squamous Cell Carcinoma, Krebsstammzelle, CSC cell, Neoplastic Stem Cell, Colony Forming Units, Oral Tongue Squamous Cell Carcinoma, Head And Neck Squamous Cell Carcinomas, Oral Squamous Cell Carcinomas, Tumor Initiating Cell, Neoplastic Colony-Forming, Squamous Cell Carcinoma of the Larynx., Oral Cavity Squamous Cell Carcinoma, Tumor Stem Cell, Squamous Cell Carcinoma of Larynx, Cells, Cancer Stem Cells, Neoplastic Colony-Forming Unit, Tumor Initiating, Head and Neck Squamous Cell Carcinoma, cancer stem-like cell, Squamous Cell of Head and Neck, Cancer"],"name_synonyms":["Human, Modern., human being, Man (Taxonomy), Homo sapiens, man, Man, human, Modern Man"],"additional_accession":[]},"is_claimable":false,"name":"Homo sapiens","description":"Phenotypic plasticity determines cancer stem cell therapeutic resistance in oral squamous cell carcinoma I","dates":{"last_updated":"2025-09-24","first_public":"2015-11-04"},"accession":"PRJNA300869","cross_references":{"GEO":["GSE74578"],"taxon":["9606"],"PubMed":["26981578"]}}