ENAGenomicsMedical School, Institite of Pathology, Johann Wolfgang-Goethe University Frankfurt a.M.https://www.ebi.ac.uk/ena/browser/view/PRJNA302924Homo sapiensxref:PubMed:27471632Hodgkin's lymphoma (HL) is one of the most frequent hematological malignancies. Due to its extraordinary composition, few tumor cells surrounded by a reactive infiltrate, HL can be seen as an ideal model system for research focusing on tumor immunology. In fact, the tumor cells of HL, so called Hodgkin/Reed-Sternberg (HRS) cells attract CD4+ T cells, which then build rosettes with the HRS cells. HRS cells further modulate the tumor microenvironment with the help of CD4+ T cells to avoid tumor rejection. Here, we mimicked this scenario using compatible CD4+ T cells receiveing data of profound interactions for the first time, as former studies were performed with allogeneic donors. Finally, we genetically retargeted compatible CD4+ T cells to kill HRS cells. Overall design: 19 samples of 7 day-old co-cultures of L428 cells and primary T cells were MACS-depleted of T cells. When indicated addition of 0.75µM dexamethasone.ENAMalignant Neoplasm, T-cell surface antigen T4/Leu-3, Malignancy, T-cell surface antigen T4|Leu-3, Neoplasms, T-cell surface glycoprotein CD4, infiltrating, Sternberg Reed Cells, Benign Neoplasm, Sternberg-Reed., Benign Neoplasms, CD4mut, Cancers, Tumor, Malignant, Cell, Malignant Neoplasms, Sternberg-Reed Cells, Neoplasias, L3T4, Benign, T-cell differentiation antigen L3T4, Cells, Neoplasm, Ly-4, CD4, Reed Sternberg Cells, Malignancies, other neoplasm, Neoplasia, Reed-Sternberg, Cancer, TumorsHuman, Modern., human being, Man (Taxonomy), Homo sapiens, man, Man, human, Modern ManfalseHomo sapiensTumor infiltrating HLA-matched CD4+ T cells retargeted against Hodgkin and Reed-Sternberg cells2025-09-242017-04-19PRJNA302924GSE75267960627471632