{"database":"ENA","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Fastqsanger.gz":["ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR299/005/SRR2993035/SRR2993035.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR299/003/SRR2993033/SRR2993033_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR299/004/SRR2993034/SRR2993034.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR299/002/SRR2993032/SRR2993032_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR299/003/SRR2993033/SRR2993033_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR299/002/SRR2993032/SRR2993032_2.fastq.gz"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Genomics"],"center_name":["John Innes Centre"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJNA305860"],"scientific_name":["Arabidopsis thaliana"],"tag":["xref:PubMed:26721863"],"long_description":["Nuclear-localized RNA binding proteins are involved in various aspects of RNA metabolism, which in turn modulates gene expression. However, the functions of nuclear-localized RNA binding proteins in plants are poorly understood. Here we report the functions of two proteins containing RNA recognition motifs, At RZ-1B and At RZ-1C, in Arabidopsis. At RZ-1B and At RZ-1C were localized to nuclear speckles and interacted with a spectrum of serine/arginine-rich (SR) proteins through their C-termini. At RZ-1C preferentially bound to purine-rich RNA sequences in vitro through its N-terminal RNA recognition motif. Disrupting the RNA-binding activity of At RZ-1C with SR proteins through over-expression of the C-terminus of At RZ-1C conferred defective phenotypes similar to those observed in At rz-1b/At rz-1c double mutants, including delayed seed germination, reduced stature, and serrated leaves. Loss of function of At RZ-1B and At RZ-1C was accompanied by defective splicing of many genes and global perturbation of gene expression. In addition, we found that At RZ-1C directly targeted FLC, promoting efficient splicing of FLC introns and likely also repressing FLC transcription. Our findings highlight the critical role of At RZ-1B/1C in regulating RNA splicing, gene expression, and many key aspects of plant development via interaction with proteins including SR proteins. Overall design: mRNA-seq to look at the transcriptome and splicing differences between wild type and At rz-1b At rz-1c mutant of Arabidopsis thaliana"],"repository":["ENA"],"additional_accession":[]},"is_claimable":false,"name":"Arabidopsis thaliana","description":"mRNA-seq data of 14 days old Arabidopsis seedlings in Col-0 and At rz-1b At rz-1c double mutant","dates":{"last_updated":"2025-09-24","first_public":"2015-12-15"},"accession":"PRJNA305860","cross_references":{"GEO":["GSE75959"],"taxon":["3702"],"PubMed":["26721863"]}}