ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR370/004/SRR3707274/SRR3707274_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR370/000/SRR3707270/SRR3707270_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR370/002/SRR3707272/SRR3707272_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR370/002/SRR3707272/SRR3707272_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR370/003/SRR3707273/SRR3707273_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR370/001/SRR3707271/SRR3707271_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR370/005/SRR3707275/SRR3707275_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR370/004/SRR3707274/SRR3707274_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR370/000/SRR3707270/SRR3707270_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR370/003/SRR3707273/SRR3707273_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR370/001/SRR3707271/SRR3707271_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR370/005/SRR3707275/SRR3707275_2.fastq.gzprimaryOK200GenomicsMFRC Room 6061, Medicine, Medical College of Wisconsinhttps://www.ebi.ac.uk/ena/browser/view/PRJNA326332Mus musculusxref:PubMed:27500496Using an experimental model of graft versus host disease (GVHD) to examine T cell-mediated inflammation within the colon, we identified a unique CD4+ T cell population that constitutively expresses the β2 integrin, CD11c, has a biased central memory phenotype and memory T cell transcriptional profile, possesses innate-like properties by gene expression analysis, and has increased expression of the gut-homing molecules, α4β7 and CCR9. Using a number of complementary GVHD mouse models, we show that adoptive transfer of these cells results in TH1-mediated proinflammatory cytokine production, augmented pathological damage in the colon, and increased mortality due to early accumulation of these cells in the GI tract. The pathogenic effects of this CD4+ T cell population was critically dependent upon co-expression of the IL-23 receptor which was required for maximal inflammatory effects. Colonic inflammation was regulated by IL-10 that was produced by non-Foxp3-expressing CD4+ T cells which attenuated lethality in the absence of functional CD4+ Foxp3+ T cells. Thus, coordinate expression of CD11c and the IL-23R defines a novel IL-10 regulated, colitogenic memory CD4+ T cell subset that is poised to initiate inflammation when there is loss of tolerance and breakdown of mucosal barriers as occurs in GVHD as well as other immune-mediated inflammatory bowel disorders. Overall design: Examination of the transcriptional profile of a novel population of CD4+ CD11c+ T cells that had a central memory T cell phenotypeENAgraft-VS-host diseases, Disease, Thymus-Dependent Lymphocytes, T-cell surface antigen T4/Leu-3, T-cell surface antigen T4|Leu-3, Graft-vs-Host Disease., T-Lymphocyte, Cell, Homologous Wasting Disease, homologous wasting disease, School-Age, L3T4, Graft-Versus-Host Diseases, T Lymphocyte, T-cell differentiation antigen L3T4, T-Cell, School Age, Diseases, Ly-4, immature T cell, T lymphocyte, graft-versus-host disease, School-Age Populations, Populations, T-cell, Graft-Versus-Host Disease, Graft Versus Host Disease, T-Cells, T-cell surface glycoprotein CD4, Runt, susceptibility to, Thymus-Dependent, homologous wasting, Graft-Versus-Host, T, CD4mut, Lymphocytes, T-lymphocyte, Population, Graft-vs-Host Diseases, School Age Populations, Homologous Wasting, Thymus-Dependent Lymphocyte, Runt Disease, Graft-vs-Host, T Cells, disease, T cell, resistance to, mature T cell, Cells, graft-versus-host diseases, CD4, T Cell, School-Age Population, School Age Population, T Lymphocytes, Thymus Dependent Lymphocytes, runt, LymphocyteMus musculus, Laboratory Mice., House, Mus, Laboratory, Swiss, Mus domesticus, mouse, Mus musculus domesticus, Swiss Mouse, mouse <Mus musculus>, Mouse, House Mice, Swiss Mice, house mouse, Mice, Laboratory Mouse, House Mouse, mice C57BL/6xCBA/CaJ hybrid, domesticus, Mus muscarisfalseMus musculusIdentification of a colitogenic memory CD4+ T cell population that mediates gastrointestinal GVHD2025-09-242016-08-13PRJNA326332GSE835521009027500496