ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR441/003/SRR4415793/SRR4415793.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR441/005/SRR4415795/SRR4415795.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR441/007/SRR4415787/SRR4415787.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR441/004/SRR4415794/SRR4415794.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR441/000/SRR4415790/SRR4415790.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR441/008/SRR4415788/SRR4415788.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR441/002/SRR4415792/SRR4415792.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR441/001/SRR4415791/SRR4415791.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR441/009/SRR4415789/SRR4415789.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR441/006/SRR4415796/SRR4415796.fastq.gzprimaryOK200GenomicsMolecular Virology, Centre of Excellence in Molecular Biology (CEMB), University of the Punjabhttps://www.ebi.ac.uk/ena/browser/view/PRJNA347838Homo sapiensAlthough several dysregulated miRNAs have been reported in liver diseases of different etiologies, no genome-wide analyses of hepatic miRNAs from patients with chronic hepatitis C (CHC) of HCV genotype 3 have been reported. With the aim of determining miRNAs associated with CHC pathogenesis, we present a comprehensive catalogue of the hepatic miRNAome of CHC-infected and control liver tissues obtained using next-generation sequencing. The study design was divided into discovery and validation phases. In the initial NGS-based discovery phase, 10 liver tissues (CHC-positive: 8, controls: 2) were subjected to miRNA-sequencing, using illumina HiSeq 2000. The expression of selected deregulated miRNAs was validated using qRT-PCR in a validation cohort comprising of 123 treatment-naive CHC patients of the HCV genotype 3 and 60 healthy controls. Furthermore, a comprehensive computational workflow incorporating miRNA–mRNA interaction analysis, was established to determine the functional significance of dysregulated miRNA–mRNA pairs in CHC infection. Overall design: 10 liver tissues (CHC-positive: 8, controls: 2) were subjected to miRNA-sequencing, using illumina HiSeq 2000ENAfalseHomo sapiensGenome-wide hepatic miRNAome profiling in chronic hepatitis C patients using next-generation sequencing [miRNA-seq]2025-09-242016-10-21PRJNA347838GSE878439606