{"database":"ENA","file_versions":[],"scores":null,"additional":{"omics_type":["Genomics"],"center_name":["Retrovirus Lab, Molecular and Comparative Pathobiology, Johns Hopkins University School of Medicine"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJNA348755"],"scientific_name":["Homo sapiens"],"tag":["xref:PubMed:28566772"],"long_description":["This study examined microRNA expression of cells maintained in media prepared with replete serum (EVR) or with serum depleted of extracellular vesicles (EVs) by either of two methods: standard overnight ultracentrifugation (UC-EVD) or a proprietary method used by Thermo Fisher (TF-EVD). Overall design: Human donor monocyte-derived macrophages, T-lymphocytic ACH-2 cells (HIV latency model), and promonocytic U1 cells (HIV-latency model) were maintained in the respective media, with the harvested media conditioned for 48 hours before harvesting. Total RNA was extracted, and miRNA profiles were generated for each sample by custom TaqMan low-density array (TLDA, Thermo Fisher)."],"repository":["ENA"],"name_synonyms":["Human, Modern., human being, Man (Taxonomy), Homo sapiens, man, Man, human, Modern Man"],"description_synonyms":["pri miRNA, RNA, miRNAs, Small Temporal, Exovesicles, Micro, Apoptotic Body, Bodies, Temporal RNA, Blood, stRNA, Apoptotic Bodies, Vesicles, Small Temporal RNA, Primary MicroRNA, Extracellular Vesicle, Primary, Blood Serum, Serum, miRNA, Primary miRNA, Cultured, INSDC_feature:ncRNA, pri-miRNA, Body, Cell, microparticle, Vesicle, Small, Micro RNA, Cultured Cell, Extracellular, Apoptotic, MicroRNA, pre-miRNA, Cultured Cells., Exovesicle, pre miRNA, Serums"],"additional_accession":[]},"is_claimable":false,"name":"Homo sapiens","description":"Effect of serum extracellular vesicle depletion protocols on miRNA expression of cultured cells","dates":{"last_updated":"2025-09-24","first_public":"2016-10-19"},"accession":"PRJNA348755","cross_references":{"GEO":["GSE88838"],"taxon":["9606"],"PubMed":["28566772"]}}