ENAGenomicsChromatin & Gene Expression Group, Cancer and Genomic Sciences, University of Birminghamhttps://www.ebi.ac.uk/ena/browser/view/PRJNA350585Mus musculusThe KMT2A/MLL1 lysine methyltransferase complex is an epigenetic regulator of selected developmental genes, in part through the SET-domain-catalyzed methylation of H3K4. It is essential for normal embryonic development and haematopoiesis and frequently mutated in cancer. The catalytic properties and targeting of KMT2A/MLL1 depend on the proteins with which it complexes and the post-translational protein modifications which some of these proteins put in place, though detailed mechanisms remain unclear. We have shown that KMT2A/MLL1 (both native and FLAG-tagged) and Msk1 (RPS6KA5), co-immunoprecipitated in various cell types. This experiment showed that the great majority of genes whose activity changed on KTM2A/MLL1 knockdown responded comparably to Msk1 knockdown. Overall design: A 10 array study in which KMT2A (Mll1) or MSK1 were knocked down in mouse embryonic fibroblasts. There were 3 control (mock transfected) samples, 4 KMT2A shRNA samples and 3 MSK1 shRNA samples.xref:PubMed:27895715ENAMus musculus, Laboratory Mice., House, Mus, Laboratory, Swiss, Mus domesticus, mouse, Mus musculus domesticus, Swiss Mouse, mouse <Mus musculus>, Mouse, House Mice, Swiss Mice, house mouse, Mice, Laboratory Mouse, House Mouse, mice C57BL/6xCBA/CaJ hybrid, domesticus, Mus muscaris3110005L17Rik, HTRX1, Laboratory, Mll, MLL, HRX, Mus domesticus, mouse, RLPK, Gene, MLL|GAS7, MLL/GAS7, House Mouse, MLL1A, mice C57BL/6xCBA/CaJ hybrid, Cxxc7, Mus muscaris, House, Mus, Mus musculus domesticus, Mice, Embryonic, TET1-MLL, Mus musculus, 6330404E13Rik, Gene Expressions, MSK1, Msk1, Swiss, mice, CXXC7, All1, WDSTS, Swiss Mouse, ALL-1, MSPK1, House Mice, Swiss Mice, Expressions, mKIAA4050, Fibroblast., domesticus, Laboratory Mice, Mll1, MLL1, 6430520K01, AI854034, TRX1, Mouse, Expression, house mouse, Laboratory MousefalseMus musculusComparison of gene expression changes following knockdown of KMT2A and MSK1 in mouse embryonic fibroblasts2025-09-242016-10-27PRJNA350585GSE891411009027895715