{"database":"ENA","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Fastqsanger.gz":["ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR504/009/SRR5040989/SRR5040989.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR504/000/SRR5040990/SRR5040990.fastq.gz"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Genomics"],"center_name":["immunobiology lab, College of Veterinary Medicine, China Agricultural University"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJNA354422"],"scientific_name":["Gallus gallus"],"long_description":["To evaluate the change of miRNA expression profile in DF-1 cells in respond to Infectious bursal disease virus (IBDV) infection, Deep sequencing was performed by LC Sciences (Hangzhou, China) on DF-1 cells infected with mock or IBDV Lx strain at an MOI of 1 for 24 h. Overall design: DF-1 cells (immortal chicken embryo fibroblast) were cultured in 100mm cell culture dish in Dulbecco modified Eagle medium (DMEM) (Invitrogen, USA) supplemented with 10% fetal bovine serum (FBS) in a 5% CO2 incubator. when cells would be 70-90% confluent, they were infected with Mock or IBDV Lx strain at an MOI of 1, and cultured in DMEM supplemented with 2% FBS . Twenty-four hours after infection, total RNA was extracted using the TRIzol reagent (LC Science, China) following the manufacturer’s protocol. The deep sequencing were performed by LC Science (hangzhou, China)."],"tag":["xref:PubMed:29564226","xref:PubMed:29046449"],"repository":["ENA"],"description_synonyms":["pri miRNA, Small, RNA, miRNAs, Small Temporal, Micro RNA, determination, Micro, Temporal RNA, chemical analysis, stRNA, Small Temporal RNA, MicroRNA, Cell., assay, Primary MicroRNA, Primary, miRNA, Primary miRNA, pre-miRNA, pre miRNA, INSDC_feature:ncRNA, pri-miRNA"],"name_synonyms":["chickens, chicken, bantam, Gallus domesticus, Gallus gallus., Chicken, Gallus gallus domesticus, Phasianus gallus"],"additional_accession":[]},"is_claimable":false,"name":"Gallus gallus","description":"Deep sequencing analysis of differences in miRNA expression between IBDV-infected and Mock-infected DF-1 cells","dates":{"last_updated":"2025-09-24","first_public":"2017-10-14"},"accession":"PRJNA354422","cross_references":{"GEO":["GSE90095"],"taxon":["9031"],"PubMed":["29046449","29564226"]}}