ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR504/009/SRR5040989/SRR5040989.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR504/000/SRR5040990/SRR5040990.fastq.gzprimaryOK200Genomicsimmunobiology lab, College of Veterinary Medicine, China Agricultural Universityhttps://www.ebi.ac.uk/ena/browser/view/PRJNA354422Gallus gallusTo evaluate the change of miRNA expression profile in DF-1 cells in respond to Infectious bursal disease virus (IBDV) infection, Deep sequencing was performed by LC Sciences (Hangzhou, China) on DF-1 cells infected with mock or IBDV Lx strain at an MOI of 1 for 24 h. Overall design: DF-1 cells (immortal chicken embryo fibroblast) were cultured in 100mm cell culture dish in Dulbecco modified Eagle medium (DMEM) (Invitrogen, USA) supplemented with 10% fetal bovine serum (FBS) in a 5% CO2 incubator. when cells would be 70-90% confluent, they were infected with Mock or IBDV Lx strain at an MOI of 1, and cultured in DMEM supplemented with 2% FBS . Twenty-four hours after infection, total RNA was extracted using the TRIzol reagent (LC Science, China) following the manufacturer’s protocol. The deep sequencing were performed by LC Science (hangzhou, China).xref:PubMed:29564226xref:PubMed:29046449ENApri miRNA, Small, RNA, miRNAs, Small Temporal, Micro RNA, determination, Micro, Temporal RNA, chemical analysis, stRNA, Small Temporal RNA, MicroRNA, Cell., assay, Primary MicroRNA, Primary, miRNA, Primary miRNA, pre-miRNA, pre miRNA, INSDC_feature:ncRNA, pri-miRNAchickens, chicken, bantam, Gallus domesticus, Gallus gallus., Chicken, Gallus gallus domesticus, Phasianus gallusfalseGallus gallusDeep sequencing analysis of differences in miRNA expression between IBDV-infected and Mock-infected DF-1 cells2025-09-242017-10-14PRJNA354422GSE9009590312904644929564226