ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR523/004/SRR5237284/SRR5237284.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR523/006/SRR5237286/SRR5237286.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR523/005/SRR5237285/SRR5237285.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR523/009/SRR5237289/SRR5237289.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR523/008/SRR5237288/SRR5237288.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR523/000/SRR5237290/SRR5237290.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR523/007/SRR5237287/SRR5237287.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR523/003/SRR5237283/SRR5237283.fastq.gzprimaryOK200GenomicsDepartment of Pharmacology, College of Medicine, National Cheng Kung Universityhttps://www.ebi.ac.uk/ena/browser/view/PRJNA371739Homo sapiensxref:PubMed:30258097ZNF322A, a C2H2 zinc finger transcription factor, is an oncoprotein in lung cancer. However, the transcription mechanisms of ZNF322A in lung cancer stemness remain elusive. By integrating our chromatin immunoprecipitation-sequencing and RNA-sequencing datasets, we identified and validated transcriptional targets of ZNF322A, which significantly enriched in developmental processes. Indeed, overexpression of ZNF322A promoted self-renewal ability and increased stemness-related gene expressions in vitro and in vivo. Importantly, ZNF322A bound directly to c-Myc promoter to transcriptionally suppress c-Myc expression, which in turn increased mitochondrial oxidative phosphorylation, promoted cell motility and thus maintained lung cancer stemness properties. Clinically, ZNF322AHigh/c-MycLow expression profile was revealed as an independent factor for poor outcome of lung cancer patients. Our study provides first evidence that ZNF322A-centered transcriptome promotes lung tumorigenesis and ZNF322A acts as a transcription suppressor of c-Myc to maintain lung cancer stemness by shifting metabolism phenotype to oxidative phosphorylation. Overall design: mRNA profiles of A549 lung cancer cells treated with control or si-ZNF322A oligo were generated by RNA-sequencingENAfalseHomo sapiensOncogenic zinc finger protein ZNF322A promotes lung cancer stemness through transcriptionally suppressing c-Myc expression [RNA-Seq]2025-09-242019-09-27PRJNA371739GSE94537960630258097