{"database":"ENA","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Fastqsanger.gz":["ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR531/001/SRR5314111/SRR5314111_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR531/002/SRR5314112/SRR5314112_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR531/001/SRR5314111/SRR5314111_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR531/002/SRR5314112/SRR5314112_1.fastq.gz"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Genomics"],"center_name":["Dr. Hal Broxmeyer Lab, Microbiology & Immunology, Indiana University"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJNA378166"],"scientific_name":["Homo sapiens"],"tag":["xref:PubMed:29377004"],"long_description":["PPARγ antagonist GW9662 treatment could enhance ex vivo expansion of human cord blood hematopoietic stem and progenitor cells (HSCs/HPCs). To gain mechanistical insights into how antagonizing PPARγ promotes expansion of HSCs/HPCs, we performed RNA sequencing (RNA seq) analysis to identify genes involved in this process. Loss of function of PPARγ in CB CD34+ cells resulted in downregulation of a number of differentiation associated genes, including CD38, CD1d, HIC1, FAM20C, DUSP4, DHRS3 and ALDH1A2, suggesting that PPARγ antagonist may maintain stemness of CB CD34+ cells, at least in part by preventing differentiation. We also observed that FBP1, encoding fructose 1, 6-bisphosphatase, a negative regulator of glycolytic flux, was significantly downregulated by treating CB CD34+ cells with GW9662. Our study demonstrates that antagonizing PPARγ signaling drives ex vivo expansion of human CB HSCs/HPCs by switching on FBP1 repressed glycolysis and preventing differentiation. Overall design: Total RNA was extracted from Vehicle or GW9662 treated cord blood CD34+ cells, and then RNA sequencing analysis was performed."],"repository":["ENA"],"additional_accession":[]},"is_claimable":false,"name":"Homo sapiens","description":"RNA sequencing analyisis to identify genes regulated by GW9662 in human cord blood hematopoietic stem and progenitor cells","dates":{"last_updated":"2025-09-24","first_public":"2018-03-01"},"accession":"PRJNA378166","cross_references":{"GEO":["GSE95703"],"taxon":["9606"],"PubMed":["29377004"]}}