ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR573/000/SRR5738280/SRR5738280.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR573/007/SRR5738287/SRR5738287.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR573/006/SRR5738286/SRR5738286.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR573/008/SRR5738278/SRR5738278.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR573/002/SRR5738282/SRR5738282.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR573/001/SRR5738281/SRR5738281.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR573/009/SRR5738279/SRR5738279.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR573/004/SRR5738284/SRR5738284.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR573/003/SRR5738283/SRR5738283.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR573/005/SRR5738285/SRR5738285.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR573/007/SRR5738277/SRR5738277.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR573/008/SRR5738288/SRR5738288.fastq.gzprimaryOK200GenomicsOmid Khorram, OB-GYN, LA Biomed at UCLA medical centerhttps://www.ebi.ac.uk/ena/browser/view/PRJNA391373Homo sapiensTotal RNA was isolated from leiomyoma and paired myometrium (N=8) and samples from three pairs were subjected to RNA sequencing. After analysis, 5941 lncRNAs (2813 up- and 3128 down-regulated at ≥1.5 fold), 148 miRNAs (56 up- and 96 down-regulated at ≥1.5 fold) and 3855 mRNAs (2030 up- and 1855 down-regulated at ≥1.5 fold) were differentially expressed in leiomyomas. Using QRT-PCR we further confirmed the expression of HULC, lnc-MEG3, LINC00890, TSIX, LINC00473, lnc-KLF9-1 and lnc-POTEM-3 (lncRNA-ATB) in leiomyoma and matched myometrium (N=8). Our results presented here provide a comprehensive expression profile of lncRNAs in leiomyomas with concurrent integrated expression of miRNAs and mRNAs in leiomyomas. Overall design: Identify the expression profile of lncRNAs with concurrent assessment of miRNAs and mRNAs profiles in leiomyomas and paired myometrium using next generation RNA sequencingxref:PubMed:28587571ENALeiomyoma, leiomyomatous neoplasm, RNA, Fibromyomas, RNA Sequence Determination, Analyses, Fibroma, Determination, Fibroid, RNA Sequence Determinations, Sequence Determination, RNA Sequence, Fibroid Tumors, Fibroid Uterus, Uterus, leiomyomatous neoplasm (morphologic abnormality), fibroid, benign, fibroid tumour, Tumor, leiomyomatous tumour, fibroid tumor, Uterine Fibroids, Sequence Determinations, fibroid neoplasm, Uterine, Sequencing, leiomyoma, Determinations, Leiomyomas, Uterine Fibroma, Uterine Fibroid, RNA Sequence Analyses, Fibroids, RNA Sequence Analysis, RNA., RNA Sequencing, leiomyomatous tumor, Fibromas, Analysis, Fibromyoma, Uterine Fibromas, Tumors, Fibroid Tumor, Sequence AnalysesHuman, Modern., human being, Man (Taxonomy), Homo sapiens, man, Man, human, Modern ManfalseHomo sapiensExpression profiling of lncRNAs, miRNAs and mRNAs and their differential expression in leiomyoma using next generation RNA sequencing2025-09-242017-06-24PRJNA391373GSE100338960628587571