{"database":"ENA","file_versions":[],"scores":null,"additional":{"omics_type":["Genomics"],"center_name":["National Cancer Center Research Institute"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJNA392318"],"scientific_name":["Homo sapiens"],"long_description":["Diffuse-type gastric cancer (DGC) frequently metastasizes to the peritoneal cavity and causes malignant ascites. Although recent studies revealed that RHOA mutation and CLDN18-ARHGAP6/26 fusions are a hallmark of DGC, their roles are largely unknown. Here, we report the roles of two novel ARHGAP fusions (RP2-ARHGAP6 and OCLN-ARHGAP26) and the above CLDN18-ARHGAP26 fusion in ascites-derived DGC cell lines. A series of experiments revealed that X-ARHGAP6 /26 fusion acts as an inhibitor of RhoA and further suggested that the ARHGAP6 fusion induces cell-adhesion loss and maintains a putative ALDH-high cancer stem cell population, whereas the ARHGAP26 fusion evades apoptosis. Notably, these ARHGAP6/26 fusions were frequently found not only in the ascites of DGC but also in that of pancreatic cancer (12% and 38%, respectively). Together, these data demonstrate that ARHGAP6/26 fusions are useful for developing therapeutics and diagnostics for peritoneal metastasis control of DGC and pancreatic cancer. We used microarrays to identify gene sets regulated by RP2-ARHGAP6-centered signaling in DGC cells. Overall design: We established two ARHGAP6 shRNA-expressing clones from RP2-ARHGAP6 harbored DGC cell line (NSC-10X1aF). In addition to these clones, control shRNA-expressing NSC-10X1aF cells were also established as control. Total 6 samples (2 independent biological experiments for each cell line) were analyzed by microarray."],"repository":["ENA"],"name_synonyms":["Human, Modern., human being, Man (Taxonomy), Homo sapiens, man, Man, human, Modern Man"],"description_synonyms":["Membrane Tissue, membrane, Carcinoma, Metastasis, anchoring, Neoplasms, Metastases, malignant neoplasm of body of pancreas, pancreatic neoplasm, familial, Pancreas Cancer, integral to membrane, Neoplasm Metastases, pancreas, neoplasm metastasis, membranous organ component, Pancreas Cancers, Pancreas Neoplasm, Pancreatic Neoplasm, cancer metastasis, Metastase, Membrane Tissues, Ca tail of pancreas, Ca head of pancreas, Pancreatic Cancer, integral component of membrane, whole membrane, Neoplasm, Pancreatic Acinar, pancreatic carcinoma, Pancreatic, Cancer of the Pancreas, Pancreatic Carcinomas, Carcinomas, malignant neoplasm of tail of pancreas, tumor cell migration, pancreatic tumor, transmembrane, membrane of organ, Cancer of Pancreas, Tissues, Pancreatic Carcinoma, malignant neoplasm of head of pancreas, Acinar Carcinoma, membrane region, Tissue, metastasis, Cancers, Pancreatic Cancers, Membrane, Pancreas, protein membrane anchor, Pancreatic Acinar Carcinoma, pancreas neoplasm., Ca body of pancreas, region of membrane, PNCA, Pancreas Neoplasms, tumor metastasis, Pancreatic Acinar Carcinomas, protein-membrane adaptor activity, Acinar Carcinomas, pancreatic tumour, Cancer"],"additional_accession":[]},"is_claimable":false,"name":"Homo sapiens","description":"Roles of membrane-Anchoring ARHGAP fusions in peritoneal metastasis of gastric and pancreatic cancer.","dates":{"last_updated":"2025-09-24","first_public":"2021-01-01"},"accession":"PRJNA392318","cross_references":{"GEO":["GSE100627"],"taxon":["9606"]}}