ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR582/004/SRR5824344/SRR5824344.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR582/009/SRR5824339/SRR5824339.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR582/003/SRR5824343/SRR5824343.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR582/001/SRR5824341/SRR5824341.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR582/000/SRR5824340/SRR5824340.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR582/002/SRR5824342/SRR5824342.fastq.gzprimaryOK200GenomicsStem Cell and Cancer, Institut Municipal d'Investigacions Mèdiques, (IMIM), Hospital del Marhttps://www.ebi.ac.uk/ena/browser/view/PRJNA394141Mus musculusWe have generated a mouse model for tumor initiation carrying a mutation in APC and lacking IKKα in intestinal epithelial cells. IKKα-deficient intestinal cells primarily failed to generate adenomas, and the few adenomas arising in this background displayed a significant reduction in cell proliferation. Using an in vitro model for intestinal tumoroids (derived from adenoma initiating cells), we have performed RNA sequencing of wild type and IKKα-deficient intestinal tumoroids. This has demonstrated that epithelial IKKα controls transcription of stem cell-related genes and genes associated with proliferation and apoptosis. Overall design: RNA sequencing of IKKa WT and KO tumoroids, done in triplicatesxref:PubMed:29438366ENAMus musculus, Laboratory Mice., House, Mus, Laboratory, Swiss, Mus domesticus, mouse, Mus musculus domesticus, Swiss Mouse, mouse <Mus musculus>, Mouse, House Mice, Swiss Mice, house mouse, Mice, Laboratory Mouse, House Mouse, mice C57BL/6xCBA/CaJ hybrid, domesticus, Mus muscaris3.4.22.-, D-APC1, D-APC2, D-Axin, e-apc, Laboratory, Daxin, Neoplasms, Mus domesticus, Benign Neoplasm, apc, mini-ICE, axn, CASP-14, Tumor, Malignant, House Mouse, DP3, PPP1R46, DP2, NFKBIKA, d-axin, House, DAPC, CG6193, Mus musculus domesticus, Min, BTPS2, GSK3beta, CG1451, D-APC, Ikka, Mice, dApc, dAPC, Fbxo24, Malignancy, Swiss, mAPC, Swiss Mice, Fbx24, Chuk1, CG7926, AI047805, Neoplasias, dAPC2/E-APC, AI256658, cyclosome, Malignancies, DmelCG6193, Cancer, Tumors, Ikbka, dAxin, DmelCG1451, dApc2, Malignant Neoplasm, din, l(3)S044230, E-APC, DP2.5, IKBKA, mouse, d-APC, APC2, dAXIN, Apc1, TCF16, APC1, xapc, Benign, E-APC dAPC2, Mus, apc 1, Mini-ICE, Neoplasm, apc1, axin, apc2, IKK-alpha, d-APC2, Mus musculus., dAPC2, dAPC1, AU020952, Mus musculus, Caspase-14 subunit p10, IKK1, mice, CC1, 0442/30, Swiss Mouse, initiation, Benign Neoplasms, House Mice, Caspase-14 subunit p19, Cancers, AW124434, IKKA, MICE, Dm APC2, Dm APC1, DAxin, Malignant Neoplasms, Laboratory Mice, domesticus, GS, DmelCG7926, APC, Mouse, anaphase promoting complex, other neoplasm, Laboratory Mouse, NeoplasiafalseMus musculusIntestinal IKKa is required for tumor initiation in APC mutant mice2025-09-242018-04-19PRJNA394141GSE1014151009029438366