ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR667/007/SRR6677397/SRR6677397.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR667/006/SRR6677396/SRR6677396.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR667/002/SRR6677392/SRR6677392.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR667/003/SRR6677393/SRR6677393.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR667/001/SRR6677391/SRR6677391.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR667/005/SRR6677395/SRR6677395.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR667/004/SRR6677394/SRR6677394.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR667/000/SRR6677390/SRR6677390.fastq.gzprimaryOK200GenomicsMagnuson, Genetics, University of North Carolina at Chapel Hillhttps://www.ebi.ac.uk/ena/browser/view/PRJNA433181Mus musculusObesity in humans is associated with worsened health outcomes following infections compared to non-obese individuals. Here, we examined the effects of adipose tissue and obesity on T cell responses to virus infection in mice. Lymphocytic choriomeningitis virus (LCMV) grew to high titer in adipose tissue. Virus-specific T cells enter the adipose tissue to resolve infection but then remain as a memory population distinct from memory T cells in lymphoid tissues. Memory CD4+ and CD8+ T cells residing in adipose were abundant, accounting for roughly 10% of all virus-specific memory T cells. Diet-induced obesity increased memory T cell number in adipose and spleen. Upon re-challenge infection, memory T cells caused severe pathogenesis, leading to sharply increased lipase levels, calcification of adipose tissue, pancreatitis, and reduced survival in obese mice but not lean mice. Thus, obesity leads to a unique form of viral pathogenesis involving memory T cell-dependent adipocyte destruction and collateral damage to other tissues. Overall design: RNA-seq comparison of CD4+ T cells in spleen vs fat (with 10 mice pooled per replicate) and CD8+ T cells in spleen vs fat (with 5 mice pooled per replicate)xref:PubMed:30970254ENAfalseObesity expands a distinct population of adipose-resident T cells and increases vulnerability to infectionObesity expands a distinct population of adipose-resident T cells and increases vulnerability to infection2025-09-242019-03-21PRJNA433181GSE1102121009030970254