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Lab, Department of Biochemistry, University of Oxford"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJNA439319"],"scientific_name":["Mus musculus"],"tag":["xref:PubMed:30228260"],"long_description":["Transcriptional regulation by chromatin is a highly dynamic process directed through the recruitment and coordinated action of epigenetic modifiers and readers of these modifications. Using an unbiased proteomic approach to find interactors of H3K36me3, a modification enriched on active chromatin, here we identify PWWP2A and HDAC2 among the top interactors. PWWP2A and its paralog PWWP2B form a stable complex with NuRD subunits MTA1/2/3:HDAC1/2:RBBP4/7, but not with MBD2/3, p66α/β, and CHD3/4. PWWP2A competes with MBD3 for binding to MTA1, thus defining a new variant NuRD complex that is mutually exclusive with the MBD2/3-containing NuRD. In mESCs, PWWP2A/B is most enriched at highly transcribed genes. Loss of PWWP2A/B leads to increases in histone acetylation predominantly at highly expressed genes, accompanied by decreases in Pol II elongation. Collectively, these findings suggest a role for PWWP2A/B in regulating transcription through the fine-tuning of histone acetylation dynamics at actively transcribed genes. Overall design: In order to explore the influence of PWWP2A/B on nascent transcription, we isolated the 4sU-labelled nascent transcripts, followed by deep sequencing. Three cell lines, E14, PWWP2A KO, and PWWP2A/B double knockout, and three biological replicates."],"repository":["ENA"],"additional_accession":[]},"is_claimable":false,"name":"Nascent transcription of E14, PWWP2AKO, and PWWP2A/B DKO","description":"Nascent transcription of E14, PWWP2AKO, and PWWP2A/B DKO","dates":{"last_updated":"2025-09-24","first_public":"2018-07-21"},"accession":"PRJNA439319","cross_references":{"GEO":["GSE112112"],"taxon":["10090"],"PubMed":["30228260"]}}