{"database":"ENA","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Fastqsanger.gz":["ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR687/006/SRR6875876/SRR6875876_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR687/003/SRR6875873/SRR6875873_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR687/004/SRR6875874/SRR6875874_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR687/005/SRR6875875/SRR6875875_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR687/006/SRR6875876/SRR6875876_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR687/005/SRR6875875/SRR6875875_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR687/004/SRR6875874/SRR6875874_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR687/003/SRR6875873/SRR6875873_2.fastq.gz"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Genomics"],"center_name":["Bioinformatics Core Facility, Wellcome Centre for Cell Biology, University of Edinburgh"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJNA442545"],"scientific_name":["Saccharomyces cerevisiae"],"tag":["xref:PubMed:31031198"],"long_description":["ChIP-seq profile of Sgo1 binding sites arrested in metaphase I by deletion of the APC/C activator CDC20. The aim of this experiment is to identify whether Sgo1 binding differs in wild type cells and cells deficient of the meiosis I-specific protein Spo13, which is required for retention of pericentromeric cohesin after metaphase I. Overall design: Diploid S. cerevisiae SK1 cells were constructed containing Sgo1-6Ha in an pGAL1-NDT80 pGPD1-GAL4.ER pCLB2-CDC20 background. Cells were allowed to sporulate in sporulation media for 5 hours before addition of β-estradiol to release from prophase arrest. Cells were grown for 75min to arrest in metaphase I after which formaldehyde was added to the cultures and cells were fixed for 30min. We chose this double-arrest strategy (with a prophase I arrest followed by a metaphase I arrest) to ensure efficient arrest in metaphase I, which is less robust in cells without Spo13. Samples were subsequently processed according to the standard lab ChIP-Seq protocol."],"repository":["ENA"],"additional_accession":[]},"is_claimable":false,"name":"Genome-wide binding of the cohesin protector Sgo1 in the presence and absence of the meiosis I-specific Spo13 protein","description":"Genome-wide binding of the cohesin protector Sgo1 in the presence and absence of the meiosis I-specific Spo13 protein","dates":{"last_updated":"2025-09-24","first_public":"2019-04-26"},"accession":"PRJNA442545","cross_references":{"GEO":["GSE112167"],"taxon":["4932"],"PubMed":["31031198"]}}