ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR687/006/SRR6875876/SRR6875876_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR687/003/SRR6875873/SRR6875873_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR687/004/SRR6875874/SRR6875874_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR687/005/SRR6875875/SRR6875875_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR687/006/SRR6875876/SRR6875876_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR687/005/SRR6875875/SRR6875875_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR687/004/SRR6875874/SRR6875874_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR687/003/SRR6875873/SRR6875873_2.fastq.gzprimaryOK200GenomicsBioinformatics Core Facility, Wellcome Centre for Cell Biology, University of Edinburghhttps://www.ebi.ac.uk/ena/browser/view/PRJNA442545Saccharomyces cerevisiaexref:PubMed:31031198ChIP-seq profile of Sgo1 binding sites arrested in metaphase I by deletion of the APC/C activator CDC20. The aim of this experiment is to identify whether Sgo1 binding differs in wild type cells and cells deficient of the meiosis I-specific protein Spo13, which is required for retention of pericentromeric cohesin after metaphase I. Overall design: Diploid S. cerevisiae SK1 cells were constructed containing Sgo1-6Ha in an pGAL1-NDT80 pGPD1-GAL4.ER pCLB2-CDC20 background. Cells were allowed to sporulate in sporulation media for 5 hours before addition of β-estradiol to release from prophase arrest. Cells were grown for 75min to arrest in metaphase I after which formaldehyde was added to the cultures and cells were fixed for 30min. We chose this double-arrest strategy (with a prophase I arrest followed by a metaphase I arrest) to ensure efficient arrest in metaphase I, which is less robust in cells without Spo13. Samples were subsequently processed according to the standard lab ChIP-Seq protocol.ENAfalseGenome-wide binding of the cohesin protector Sgo1 in the presence and absence of the meiosis I-specific Spo13 proteinGenome-wide binding of the cohesin protector Sgo1 in the presence and absence of the meiosis I-specific Spo13 protein2025-09-242019-04-26PRJNA442545GSE112167493231031198