ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR687/000/SRR6876050/SRR6876050_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR687/001/SRR6876051/SRR6876051_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR687/008/SRR6876048/SRR6876048_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR687/001/SRR6876051/SRR6876051_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR687/000/SRR6876050/SRR6876050_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR687/009/SRR6876049/SRR6876049_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR687/009/SRR6876049/SRR6876049_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR687/008/SRR6876048/SRR6876048_1.fastq.gzprimaryOK200GenomicsBioinformatics Core Facility, Wellcome Centre for Cell Biology, University of Edinburghhttps://www.ebi.ac.uk/ena/browser/view/PRJNA442754Saccharomyces cerevisiaexref:PubMed:31031198ChIP-seq profile of Spo13 binding sites arrested in prophase I by deletion of the meiotic transcription factor NDT80. Previous ChIP-ChIP data had indicated that Spo13 binding correlates with cohesin binding sites. Therefore, in addition to wild type cells, we also investigated cells deficient of the meiosis-specific cohesin subunit Rec8 to identify cohesin-dependent binding sites of Spo13. Overall design: Diploid S. cerevisiae SK1 cells were constructed containing Spo13-3Flag in an ndt80Δ background. Cells were allowed to sporulate in sporulation media for 5 hours after which formaldehyde was added to the cultures and cells were fixed for 30min. Samples were then processed according to the standard lab ChIP-Seq protocol.ENASPNGAF60, meiosis I nuclear division, wide, count in organism, absence, absent from organism., wide/broad, Genomes, ligand, number, whole genome, broad, presenceSPNGAF60, meiosis I nuclear division, wide, count in organism, absence, absent from organism., wide/broad, Genomes, ligand, number, whole genome, broad, presencefalseGenome-wide binding of the meiosis I-specific Spo13 in the presence and absence of cohesinGenome-wide binding of the meiosis I-specific Spo13 in the presence and absence of cohesin2025-09-242019-04-26PRJNA442754GSE112170493231031198