ENAGenomicsUniversity of Oklahomahttps://www.ebi.ac.uk/ena/browser/view/PRJNA445726Leptuca pugilatorThe intermolt crustacean Y-organ (YO) maintains a basal state mediated by high circulating levels of molt inhibiting hormone (MIH), a neuropeptide inhibiting YO ecdysteroidogenesis. During early premolt, the YO undergoes activation, characterized by reduced circulating MIH. In the fiddler crab, molt cycle entry was induced via Limb Bud Autotomy (LBA).Limb bud tissues were harvested at 1, 3, and 7 days post-ESA and processed for RNA-seq. mRNA levels for Growth signalling pathway genes were elevated over intermolt animals, but declined following rapamycin treatment. In concert with mTOR inhibition, ecdysteroid pathway genes decreased in mRNA expression, accompanied by decreases in ecdysteroid titer. On entering premolt, decreases in mRNA abundance were also observed in cyclic nucleotide gene pathway components, supporting earlier work indicating these regulatory pathways mediate MIH signaling and genes associated with Wnt and Insulin-like signaling pathways, suggesting both inhibitory and stimulatory transcriptional effects mediated by YO mTOR pathway inhibition.ENAfalseLeptuca pugilator breed:Eukaryote | cultivar:Consejo Dominicano de Pescay Acuicultura, Dominican RepublicLeptuca pugilator breed:Eukaryote | cultivar:Consejo Dominicano de Pescay Acuicultura, Dominican Republic Raw sequence reads2025-09-242018-03-28PRJNA4457266772