ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR691/003/SRR6912753/SRR6912753.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR691/008/SRR6912758/SRR6912758.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR691/004/SRR6912754/SRR6912754.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR691/006/SRR6912756/SRR6912756.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR691/007/SRR6912757/SRR6912757.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR691/005/SRR6912755/SRR6912755.fastq.gzprimary200OKGenomicsSchool of Life Sciences, University of Science & Technology of Chinahttps://www.ebi.ac.uk/ena/browser/view/PRJNA447866Caenorhabditis elegansxref:PubMed:29791857RNAi-elicited gene silencing is heritable and can persist for multiple generations after its initial induction in C. elegans. However, the mechanism by which parental-acquired trait-specific information from RNAi is inherited by the progenies is not fully understood. Here, we identified a cytoplasmic Argonaute protein, WAGO-4, necessary for the inheritance of RNAi. WAGO-4 exhibits asymmetrical translocation to the germline during early embryogenesis, accumulates at the perinuclear foci in the germline, and is required for the inheritance of exogenous RNAi targeting both germline- and soma-expressed genes. WAGO-4 binds to 22G-RNA and its mRNA targets. Interestingly, WAGO-4-associated endogenous 22G-RNA targets the same cohort of germline genes as CSR-1 and similarly contains untemplated addition of uracil at the 3' ends. The poly(U) polymerase CDE-1 is required for the untemplated polyuridylation of WAGO-4-associated 22G-RNAs and inheritance of RNAi. Therefore, we conclude that the cytoplasmic Argonaute protein WAGO-4 also promotes the inheritance of RNAi in addition to the nuclear RNAi pathway. Overall design: In this study, we compared WAGO-4 associated small RNAs from (1) wild type animals (2) after lin-15b RNAi. And we also investigated the function of WAGO-4 in RNAi inheritance by comparing WAGO-4 associated small RNAs in (3) no RNAi treatment (4) oma-1 dsRNA treated P0 (5) F1 animals from oma-1 dsRNA-treated P0 (6) F3 animals from oma-1 dsRNA-treated P0.ENAPost-Transcriptional Gene Silencings, post-transcriptional gene silencing by RNA, RNA, Inheritance, protein complex, Posttranscriptional, Posttranscriptional Gene Silencing, Proteins, posttranscriptional gene silencing by siRNA, Gene, protein, protein-containing complex, cosuppression, quelling, Co Suppression, Cosuppression, Protein Gene Products, Gene Proteins, RNAi, Gene Silencings, Post-Transcriptional, Post Transcriptional Gene Silencing., Gene Silencing, Interference, native protein, Protein, Gene Products, Post Transcriptional, protein aggregate, Co-Suppression, Post-Transcriptional Gene Silencing, RNA Silencing, Silencing, Post-Transcriptional GenePost-Transcriptional Gene Silencings, post-transcriptional gene silencing by RNA, RNA, Inheritance, protein complex, Posttranscriptional, Posttranscriptional Gene Silencing, Proteins, posttranscriptional gene silencing by siRNA, Gene, protein, protein-containing complex, cosuppression, quelling, Co Suppression, Cosuppression, Protein Gene Products, Gene Proteins, RNAi, Gene Silencings, Post-Transcriptional, Post Transcriptional Gene Silencing., Gene Silencing, Interference, native protein, Protein, Gene Products, Post Transcriptional, protein aggregate, Co-Suppression, Post-Transcriptional Gene Silencing, RNA Silencing, Silencing, Post-Transcriptional GenefalseA cytoplasmic Argonaute protein promotes the inheritance of RNAiA cytoplasmic Argonaute protein promotes the inheritance of RNAi2025-09-242018-04-30PRJNA447866GSE112475623929791857