ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR723/007/SRR7235107/SRR7235107.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR723/009/SRR7235109/SRR7235109.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR723/004/SRR7235094/SRR7235094.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR723/002/SRR7235102/SRR7235102.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR723/003/SRR7235103/SRR7235103.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR723/007/SRR7235097/SRR7235097.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR723/005/SRR7235105/SRR7235105.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR723/000/SRR7235100/SRR7235100.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR723/005/SRR7235095/SRR7235095.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR723/001/SRR7235111/SRR7235111.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR723/008/SRR7235108/SRR7235108.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR723/000/SRR7235110/SRR7235110.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR723/001/SRR7235101/SRR7235101.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR723/008/SRR7235098/SRR7235098.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR723/006/SRR7235106/SRR7235106.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR723/006/SRR7235096/SRR7235096.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR723/009/SRR7235099/SRR7235099.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR723/004/SRR7235104/SRR7235104.fastq.gzprimaryOK200GenomicsAllergy and Immunology, CCHMChttps://www.ebi.ac.uk/ena/browser/view/PRJNA473789Homo sapiensThe aim of this study was to examine the transcriptional regulatory activity of nuclear IL-33 independent of its well-described extracellular cytokine activity mediated by its receptor, ST2. Overall design: Single-cell clones of Human TE-7 esophageal epithelial cells, which lack expression of the ST2 receptor, and with lentiviral-mediated, Dox-inducible overexpression of full-length IL-33, a non-chromatin binding form of IL-33 (amino acids 112-270), or empty vector (pINDUCER20) were treated with 100 ng/mL of doxycycline (Clontech) or control media for 48 hours. Expression of wild-type and truncated IL-33 was verified by Western blot. RNA was isolated using Tripure reagent and subjected to genome-wide RNA-sequencing through the Cincinnati Children’s Hospital Medical Center Gene Expression Core.xref:PubMed:30108214ENAfalseEffect of nuclear IL-33 on gene expressionEffect of nuclear IL-33 on gene expression2025-09-242018-06-02PRJNA473789GSE115097960630108214