ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR727/008/SRR7271178/SRR7271178_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR727/009/SRR7271189/SRR7271189_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR727/006/SRR7271176/SRR7271176_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR727/008/SRR7271188/SRR7271188_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR727/007/SRR7271177/SRR7271177_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR727/009/SRR7271179/SRR7271179_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR727/003/SRR7271183/SRR7271183_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR727/004/SRR7271184/SRR7271184_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR727/006/SRR7271186/SRR7271186_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR727/004/SRR7271174/SRR7271174_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR727/005/SRR7271185/SRR7271185_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR727/007/SRR7271187/SRR7271187_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR727/005/SRR7271175/SRR7271175_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR727/006/SRR7271186/SRR7271186_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR727/005/SRR7271185/SRR7271185_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR727/009/SRR7271179/SRR7271179_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR727/001/SRR7271181/SRR7271181_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR727/009/SRR7271189/SRR7271189_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR727/008/SRR7271188/SRR7271188_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR727/007/SRR7271187/SRR7271187_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR727/000/SRR7271180/SRR7271180_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR727/002/SRR7271182/SRR7271182_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR727/004/SRR7271174/SRR7271174_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR727/001/SRR7271181/SRR7271181_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR727/006/SRR7271176/SRR7271176_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR727/000/SRR7271180/SRR7271180_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR727/005/SRR7271175/SRR7271175_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR727/008/SRR7271178/SRR7271178_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR727/002/SRR7271182/SRR7271182_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR727/004/SRR7271184/SRR7271184_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR727/003/SRR7271183/SRR7271183_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR727/007/SRR7271177/SRR7271177_1.fastq.gzprimaryOK200GenomicsBGIhttps://www.ebi.ac.uk/ena/browser/view/PRJNA474776In this study, two DNA extraction methods on different sample status (snap frozen and fresh) were compared, meanwhile two different library preparation methods with different sample inputs are performed, the goal is to evaluate the optimal experimental protocols to get highest data quality for samples in different status.ENAfalseEvaluation of sample extraction and library preparation for short-gun metagenomics2023-05-192018-07-31PRJNA474776