ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR751/000/SRR7512050/SRR7512050_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR751/001/SRR7512051/SRR7512051_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR751/004/SRR7512044/SRR7512044_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR751/005/SRR7512045/SRR7512045_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR751/009/SRR7512049/SRR7512049_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR751/000/SRR7512050/SRR7512050_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR751/008/SRR7512048/SRR7512048_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR751/006/SRR7512046/SRR7512046_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR751/007/SRR7512047/SRR7512047_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR751/005/SRR7512045/SRR7512045_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR751/008/SRR7512048/SRR7512048_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR751/009/SRR7512049/SRR7512049_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR751/001/SRR7512051/SRR7512051_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR751/006/SRR7512046/SRR7512046_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR751/004/SRR7512044/SRR7512044_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR751/007/SRR7512047/SRR7512047_1.fastq.gzprimaryOK200GenomicsInstitute of Developmental Biology, Biocenter Cologne, University of Colognehttps://www.ebi.ac.uk/ena/browser/view/PRJNA480717Arabidopsis thalianaxref:PubMed:31037302Two methods Fluorescence Activated Cell Sorting (FACS) and Assay for Transposase-Accessible Chromatin with high-throughput sequencing (ATAC-seq) are combined to analyse the chromatin accessibility of Lateral Organ Founder Cells (LOFCs) in the peripheral zone of the apetala1 cauliflower double mutant Arabidopsis inflorescence meristem. Genome-wide we observed a striking correlation between Transposase Hypersensitive Sites (THS) detected in ATAC-seq and DNase I Hypersensitive Sites (DHS), covering mostly extended regions substructured into several individual THS that correspond to phylogenetically conserved sequence, enhancer elements and binding sites of MADS-box transcription factors. Relative to available RNA-seq data, chromatin configuration changes according to gene activation or repression, i.e. at cellular resolution chromatin regions gain or lose Tn5 transposase accessibility in direct correlation with gene expression levels. A pronounced THS priority immediately upstream of the transcription start and reduced numbers of THSs in the transcription unit are complementary to established H3K4me3 activation or H3K27me3 repressive marks. At this resolution, the FACS/ATAC-seq combination should be widely applicable to detect chromatin changes in course of cell type specification and facilitate the detection of regulatory promoter elements in plant promoters. Overall design: Examination of chromatin accessibility via ATAC-seq in Lateral organ founder cells and non founder cell neighbours. Three biological replicates were performed for each cell type. Two control samples of sheared DNA from the same tissue were sequenced.ENAnuclear chromatin, HITS-CLIP, High Throughput Sequencing of RNA Isolated by Crosslinking Immunoprecipitation, measuring, cytoplasmic chromatin, ChIP-Chip, Chromatin Immuno-precipitation, determination, Arbisopsis thaliana, A., Cross Linking and Immunoprecipitation Followed by Deep Sequencing, Arabidopsis thaliana, ChIP Sequencing, CLIP-Seq, A. thaliana, Assay for Transposase-Accessible Chromatin Using Sequencing, ChIP-PET, Cell, Arabis thaliana, ChIP-Exo, element, Arabidopsis thalianas, A. thalianas, chemical analysis, Chromatin Immunoprecipitation Sequencing-Chip, thalianas, Arabidopsis thaliana (thale cress), High-Throughput Sequencing of RNA Isolated by Crosslinking Immunoprecipitation, Chromatins, organ, thale cress, mouse-ear cress, Chromatin Immunoprecipitation Sequencing Chip, Chromatin Immuno precipitation Sequencing, ATAC-seq assay, Cresses, ChIP, Chromatin Immunoprecipitation Paired End Tag, ATAC-seq, Chromatin Immuno Precipitation Paired End Tag, Mouse-ear Cress, Cross-Linking and Immunoprecipitation Followed by Deep Sequencing, thaliana, Chromatin Immunoprecipitation, Chromatin Immuno-precipitation Sequencing, ChIP Exonuclease, Mouse-ear Cresses, ChIP-Seq, Mouse-ear, Sequencing, Assay for Transposase Accessible Chromatin Using Sequencing, Chromatin Immunoprecipitation Paired-End Tag, thale-cress, Arabidopsis, anatomical unit, scientific observation, ChIA-PET., Transposase, Chromatin Immuno-Precipitation Paired-End Tag, chromosome scaffold, body organ, ATAC-Seq, Chromatin Immunoprecipitation Sequencing-Chips, Cress, assay, Mouse ear, Arabidopses, ChIP-Exonucleasenuclear chromatin, HITS-CLIP, High Throughput Sequencing of RNA Isolated by Crosslinking Immunoprecipitation, measuring, cytoplasmic chromatin, ChIP-Chip, Chromatin Immuno-precipitation, determination, Arbisopsis thaliana, A., Cross Linking and Immunoprecipitation Followed by Deep Sequencing, Arabidopsis thaliana, ChIP Sequencing, CLIP-Seq, A. thaliana, Assay for Transposase-Accessible Chromatin Using Sequencing, ChIP-PET, Cell, Arabis thaliana, ChIP-Exo, element, Arabidopsis thalianas, A. thalianas, chemical analysis, Chromatin Immunoprecipitation Sequencing-Chip, thalianas, Arabidopsis thaliana (thale cress), High-Throughput Sequencing of RNA Isolated by Crosslinking Immunoprecipitation, Chromatins, organ, thale cress, mouse-ear cress, Chromatin Immunoprecipitation Sequencing Chip, Chromatin Immuno precipitation Sequencing, ATAC-seq assay, Cresses, ChIP, Chromatin Immunoprecipitation Paired End Tag, ATAC-seq, Chromatin Immuno Precipitation Paired End Tag, Mouse-ear Cress, Cross-Linking and Immunoprecipitation Followed by Deep Sequencing, thaliana, Chromatin Immunoprecipitation, Chromatin Immuno-precipitation Sequencing, ChIP Exonuclease, Mouse-ear Cresses, ChIP-Seq, Mouse-ear, Sequencing, Assay for Transposase Accessible Chromatin Using Sequencing, Chromatin Immunoprecipitation Paired-End Tag, thale-cress, Arabidopsis, anatomical unit, scientific observation, ChIA-PET., Transposase, Chromatin Immuno-Precipitation Paired-End Tag, chromosome scaffold, body organ, ATAC-Seq, Chromatin Immunoprecipitation Sequencing-Chips, Cress, assay, Mouse ear, Arabidopses, ChIP-ExonucleasefalseChromatin changes in lateral organ founder cells of Arabidopsis thaliana detected by assay for transposase-accessible chromatin sequencing (ATAC-seq)Chromatin changes in lateral organ founder cells of Arabidopsis thaliana detected by assay for transposase-accessible chromatin sequencing (ATAC-seq)2025-09-242019-04-04PRJNA480717GSE116972370231037302