ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR763/000/SRR7631670/SRR7631670.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR763/005/SRR7631685/SRR7631685.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR763/003/SRR7631683/SRR7631683.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR763/001/SRR7631681/SRR7631681.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR763/008/SRR7631668/SRR7631668.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR763/009/SRR7631679/SRR7631679.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR763/005/SRR7631675/SRR7631675.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR763/008/SRR7631688/SRR7631688.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR763/004/SRR7631674/SRR7631674.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR763/009/SRR7631669/SRR7631669.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR763/002/SRR7631672/SRR7631672.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR763/000/SRR7631680/SRR7631680.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR763/007/SRR7631677/SRR7631677.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR763/001/SRR7631671/SRR7631671.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR763/007/SRR7631667/SRR7631667.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR763/008/SRR7631678/SRR7631678.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR763/006/SRR7631686/SRR7631686.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR763/002/SRR7631682/SRR7631682.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR763/009/SRR7631689/SRR7631689.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR763/006/SRR7631676/SRR7631676.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR763/007/SRR7631687/SRR7631687.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR763/000/SRR7631690/SRR7631690.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR763/003/SRR7631673/SRR7631673.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR763/004/SRR7631684/SRR7631684.fastq.gzprimaryOK200GenomicsSladek Lab, Molecular, Cell and Systems Biology, University of California Riversidehttps://www.ebi.ac.uk/ena/browser/view/PRJNA483880Mus musculusHepatocyte Nuclear Factor 4α (HNF4α), master regulator of hepatocyte differentiation, is regulated by two promoters (P1 and P2). P1-HNF4α but not P2-HNF4α is expressed in normal adult liver while both P1- and P2-HNF4α are expressed in fetal liver and liver cancer. To determine the physiological function of the HNF4α isoforms, we compared P2-HNF4α-expressing exon swap mice to wildtype (WT) using RNA-seq, ChIP-seq, proteomics, protein binding microarrays (PBMs) and metabolomics. P2-HNF4α orchestrates a distinct transcriptomic and metabolomic profile. Overall design: Liver RNA-seq samples from both genotypes (WT and a7HMZ, P1- and P2-HNF4α respectively) were taken at three time-points as well as a 12-hr fast with both genotypes at the earliest time-point. Please note that three different time points are indicated in the sample titles as the time of the day, in 24hr notation (i.e. 1030 = 10:30am, 1330 = 1:30pm, and 2030 = 8:30pm).ENAfalseDevelopmentally regulated isoform of HNF4α reprograms the adult liverDevelopmentally regulated isoform of HNF4α reprograms the adult liver2025-09-222018-09-03PRJNA483880GSE11797210090