{"database":"ENA","file_versions":[],"scores":null,"additional":{"omics_type":["Genomics"],"center_name":["Developmental Morphogenesis, Graduate School of Science, Nagoya University"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJNA486330"],"scientific_name":["Mus musculus"],"long_description":["The free limb is subdivided into three anatomical domains along the proximodistal axis: the stylopod, zeugopod and autopod. These domains possess a specific number and pattern of the bones and each bone possesses unique morphology and length. During limb development, the mesenchymal cells of limb bud first forms precartilaginous aggregates which eventually grow and differentiate into the limb cartilage. Expression of Hox genes belonging to the Abd-B family, Hox9-13, is closely related to the anatomical domains of the limb bones and crucial for controlling morphogenesis and patterning of the limb cartilage. Hoxa13 and Hoxd13 are expressed in the autopodal mesenchyme and double KO mice have smaller autopods during the embryonic stage and a fan-shaped cartilage develops instead of the normal five digits. Thus, Hoxa13 and Hoxd13 are crucial for the cartilage patterning in the autopod. Since Hox encodes a transcription factor, Hox target genes function in region/compartment-specific formation of the precartilaginous condensation, cellular differentiation and proliferation of the cartilage. We used microarray to identified downstream genes of Hox13 in the autopod as exhibiting changes in the expression in the Hox13dKO mutant embryos. Overall design: Hoxa13 and Hoxd13 show overlapping expression in the autopodal mesenchyme and cooperatively function in autopodal cartilage pattern formation. Hoxd11 and Hoxd12 are also expressed in the autopodal mesenchyme and cooperate in autopodal cartilage pattern formation in a gene dosage-dependent manner together with Hoxa13 and Hoxd13. Specifically, homozygote with simultaneous deletion of Hoxd11,12,13 (HoxDdel) exhibited more severe adult cartilage phenotypes than Hoxd13 KO homozygotes. To increase the sensitivity in analyzing the Hox function we used Hoxa13-/- HoxDdel/del double homozygotes (Hox13dKO). We isolated RNA from the autopod around E11.25 wild type and Hox13dKO embryos and quantified the transcript levels by Genechip. In the wild-type autopod at E11.25, Hoxa13 and Hoxd13 are expressed in the mesenchyme. At this stage, the precartilaginous condensation of the carpal/tarsal region is visible and the precartilaginous condensation of metacarpal/metatarsal region begin to form as protrusions from the carpal/tarsal condensation. We isolated the autopodal tissue corresponding to the Hoxd13 expressing region from both fore- and hindlimb buds."],"tag":["xref:PubMed:30895612"],"repository":["ENA"],"name_synonyms":["Gene., Gene, Expression, Gene Expressions, Expressions"],"description_synonyms":["Gene., Gene, Expression, Gene Expressions, Expressions"],"additional_accession":[]},"is_claimable":false,"name":"Alteration of the gene expression profile in the Hox13dKO","description":"Alteration of the gene expression profile in the Hox13dKO","dates":{"last_updated":"2025-09-24","first_public":"2019-03-30"},"accession":"PRJNA486330","cross_references":{"GEO":["GSE118640"],"taxon":["10090"],"PubMed":["30895612"]}}